INCREASED IL-6-PRODUCTION BY MONOCYTES AND KERATINOCYTES IN PATIENTS WITH PSORIASIS
INCREASED IL-6-PRODUCTION BY MONOCYTES AND KERATINOCYTES IN PATIENTS WITH PSORIASIS
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DOI：
10.1111/1523-1747.ep12477880
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发表时间：
1991-07-01
影响因子：
6.5
通讯作者：
LUGER, TA
中科院分区：
文献类型：
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作者：
NEUNER, P;URBANSKI, A;LUGER, TA
白细胞介素-6 （IL-6）是一种多功能炎性细胞因子，由单核细胞和角质形成细胞在刺激下产生。由于银屑病是一种皮肤疾病，其特征是角质形成细胞的过度增生和炎症浸润，因此本研究对银屑病患者单核细胞和角质形成细胞的IL-6产生进行了研究。来自银屑病患者、特应性和健康对照的外周血单个核细胞（PBMC）孵育24小时，随后使用IL-6依赖性杂交瘤细胞系（B9）测量上清IL-6活性。与对照组和特应性相比，银屑病患者PBMC产生的生物活性IL-6显著增加。使用针对IL-6的特异性抗血清进行Western blot分析也证实了这些发现。此外，当对同一患者的血清进行IL-6活性检测时，银屑病患者的血清中循环IL-6的含量明显高于特应性和健康对照的样本。与正常或未受损伤的皮肤相比，免疫组织化学和原位杂交检测发现，银屑病病变中的角质形成细胞IL-6明显阳性。此外，IL-6还可诱导单核细胞自身的合成和释放。这些发现表明，牛皮癣中的角化细胞和单核细胞被激活，产生增加的IL-6， IL-6可能是参与调节局部和全身炎症反应的介质之一，发生在牛皮癣等皮肤病。
Interleukin-6 (IL-6) is a multifunctional inflammatory cytokine that is produced by monocytes and keratinocytes upon stimulation. Because psoriasis is a skin disease characterized by a hyperproliferative activity of keratinocytes and an inflammatory infiltrate, in the present study IL-6 production of monocytes and keratinocytes of patients with psoriasis was investigated. Peripheral blood mononuclear cells (PBMC) derived from psoriatics, atopics, and healthy controls were incubated for 24 h and, subsequently, supernatant IL-6 activity was measured using an IL-6-dependent hybridoma cell line (B9). Compared to controls and atopics, PBMC of psoriatics produced significantly increased amounts of biologically active IL-6. These findings were also confirmed by Western blot analysis using a specific antiserum directed against IL-6. Moreover, when the sera of the same patients were tested for IL-6 activity, sera of psoriatics contained significantly elevated amounts of circulating IL-6 in comparison to samples from atopics and healthy controls. In contrast to normal or uninvolved skin, keratinocytes in psoriatic lesions were remarkably positive for IL-6 as detected by immunohistochemistry and in situ hybridization. In addition, IL-6 also was found to induce its own synthesis and release by monocytes. These findings indicate that keratinocytes and monocytes in psoriasis are activated to produce increased amounts of IL-6, which may be one of the mediators involved in the regulation of both local and systemic inflammatory reactions occurring in skin diseases such as psoriasis.