Effects of different UV radiation on photoprotective pigments and antioxidant activity of the hot-spring cyanobacterium Leptolyngbya cf. fragilis
Effects of different UV radiation on photoprotective pigments and antioxidant activity of the hot-spring cyanobacterium Leptolyngbya cf. fragilis
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DOI：
10.1111/pre.12374
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发表时间：
2019-07-01
影响因子：
1.5
通讯作者：
Arman, Mitra
中科院分区：
文献类型：
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作者：
Kokabi, Maryam;Yousefzadi, Morteza;Arman, Mitra
研究了紫外光诱导温泉蓝藻Leptolyngbya cf中光保护色素的合成/积累和抗氧化活性。脆弱的。结果表明，紫外光照射可诱导藻类胡萝卜素、别藻蓝蛋白、藻红蛋白和藻红蛋白的生物合成，而藻蓝蛋白在长时间的紫外光照射下降解。此外，还研究了L.cf.随着紫外线照射次数的增加，脆弱度显著改变，这可能是由于附属色素的破坏和重新合成作为一种适应紫外线胁迫的策略。经紫外线处理的蓝藻不同提取物的体外抗氧化性分析显示出浓度依赖的抗氧化活性。紫外线处理72 h的乙酸乙酯提取物的总抗氧化活性最高(IC_(50)=71.73+/-5.3mU·g·mL~(-1))，其次是未经紫外线照射的乙酸乙酯提取物(IC_(50)=109.43+/-2.76mU·g·mL~(-1))。这是首次报道紫外光诱导合成的光保护色素及其抗氧化活性。脆弱的。
UV-induced synthesis/accumulation of photoprotective pigments and antioxidant activity were investigated in the hot-spring cyanobacterium Leptolyngbya cf. fragilis. The results indicated that UV radiation may induce biosynthesis of carotenoids, allophycocyanin, phycoerythrin, and scytonemin while phycocyanin degrades in response to longtime UV radiation. Moreover, pigment composition of L. cf. fragilis was significantly altered with increasing UV radiation times, probably due to destruction and resynthesis of accessory pigments as an adaptation strategy to UV stress. The in vitro antioxidant analysis of different extracts of UV treated cyanobacteria exhibited concentration-dependent antioxidant activity. Ethyl acetate extract of 72 h UV treatment showed maximum total antioxidant activity (IC50 = 71.73 +/- 5.3 mu g mL(-1)) followed by ethyl acetate control (non-UV irradiated) extract (IC50 = 109.43 +/- 2.76 mu g mL(-1)). This is the first report for the UV-induced synthesis of photoprotective pigments and their antioxidant activity in L. cf. fragilis.