Biocompatibility of functionalized designer self-assembling nanofiber scaffolds containing FRM motif for neural stem cells.
Biocompatibility of functionalized designer self-assembling nanofiber scaffolds containing FRM motif for neural stem cells.
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DOI：
10.1002/jbm.a.34804
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发表时间：
2014-05
期刊：
影响因子：
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通讯作者：
Zhen-wei Zou;Ting Liu;Jingfeng Li;Pin-dong Li;Q. Ding;G. Peng;Q. Zheng;Xianlin Zeng;Yong-chao Wu;Xiao-dong Guo
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文献类型：
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作者：
Zhen-wei Zou;Ting Liu;Jingfeng Li;Pin-dong Li;Q. Ding;G. Peng;Q. Zheng;Xianlin Zeng;Yong-chao Wu;Xiao-dong Guo
多肽自组装支架在组织工程中有着广泛的应用。许多工作都集中在修饰具有功能基序的自组装支架以获得所需的生物活性。我们在这里报告的发展，专门为神经组织工程（NTE）设计的生物材料。以RADA-16（AcN-RADARADARADA-CONH 2）为基本骨架，合成了含有神经细胞粘附分子（NCAM）衍生的模拟肽FRM（SIDRVEPYSSTAQ）的31个氨基酸的肽RADA-FRM（AcN-RADARADARADAGGSIDRVEPYSSTAQ-CONH 2），该肽能自组装成一个可降解的骨架。我们使用原子力显微镜（AFM）测试了纳米纤维素支架的表征，并评估了含有FRM的纳米纤维素支架（FRM-NS）的流变学性质。然后，我们研究了它对新生大鼠神经干细胞（NSCs）的生物相容性。遗憾的是，我们发现FRM-NS对神经干细胞的分化没有影响。然而，我们测试了FRM-NS无细胞毒性。此外，与纯RADA-16支架相比，我们发现设计的含FRM基序的自组装肽支架能显著促进NSCs增殖并刺激NSCs向三维支架迁移。我们的研究结果表明，新的设计师肽支架含有FRM与神经干细胞具有良好的生物相容性，并可能是有用的中枢神经组织修复。
Peptide self-assembling scaffolds have been widely used in tissue engineering. Much work has been focused on modifying the self-assembling scaffolds with functional motifs for desired biological activities. We report here the development of a biological material designed specifically for neural tissue engineering (NTE). Using RADA-16 (AcN-RADARADARADARADA-CONH2) as a base scaffold, we synthesized a 31 amino acid peptide RADA-FRM (AcN-RADARADARADARADAGGSIDRVEPYSSTAQ-CONH2) containing the neural cell adhesion molecule (NCAM)-derived mimetic peptide FRM (SIDRVEPYSSTAQ), which could undergo self-assembly into a nanofiber scaffold. We tested the characterization of the nanofiber scaffold using atomic force microscopy (AFM) and accessed the rheological properties of FRM-containing nanofiber scaffold (FRM-NS). Then we examined its biocompatibility on neural stem cells (NSCs) from neonatal rats. Regrettably, we found that FRM-NS had no effect on differentiation of NSCs. However, we tested that FRM-NS was noncytotoxic. Furthermore, compared to pure RADA-16 scaffold, we found that the designer self-assembling peptide scaffold containing FRM motif could significantly promote NSCs proliferation and stimulate NSCs migration into the three-dimensional scaffold. Our results indicate that the novel designer peptide scaffold containing FRM had excellent biocompatibility with NSCs and may be useful for central nervous tissue repair.