INTRAGASTRIC COLONIZATION OF INFANT MICE WITH CANDIDA-ALBICANS INDUCES SYSTEMIC IMMUNITY DEMONSTRABLE UPON CHALLENGE AS ADULTS
INTRAGASTRIC COLONIZATION OF INFANT MICE WITH CANDIDA-ALBICANS INDUCES SYSTEMIC IMMUNITY DEMONSTRABLE UPON CHALLENGE AS ADULTS
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DOI：
10.1093/infdis/157.5.950
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发表时间：
1988-05-01
影响因子：
6.4
通讯作者：
DOMER, JE
中科院分区：
文献类型：
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作者：
DOMER, JE
用幼年CBA/J小鼠插管建立白念珠菌长期定植小鼠模型，研究定植对成年动物白念珠菌特异性免疫应答的影响。两个显著的结果是刺激保护性免疫反应，证明是静脉注射(IV)的小鼠存活率增加和集落形成单位减少，以及引发迟发型超敏反应的T细胞成分。成年后使用细胞壁衍生抗原进行测试的定植小鼠很少或根本没有明显的水解性肝病。然而，如果皮肤接种一次可存活的念珠菌，他们会显著增强水解酶反应，这与皮肤接种无关。将相同数量的死亡有机体接种到幼鼠体内，既不能激发动物提高水解度，也不能刺激保护性免疫。在定植的动物体内可见抗念珠菌胞质抗原的抗体，用最高剂量的胚化分生孢子iv攻击的动物其抗体产量显著增加。
A murine model of long-term colonization withCandida albicans, established by intubating infant CBA/J mice, was used to study the effects of colonization on the development ofCandida-specific immune responses in mature animals. Two striking consequences were stimulation of protective immune responses, as evidenced by increased survival and decreased numbers of colony-forming units in mice challenged intravenously (iv), and priming of the T cell component responsible for delayed hypersensitivity (DH). Colonized mice, tested as adults by using a cell wall-derived antigen, had little or no demonstrable DH. If inoculated once cutaneously with viableCandida, however, they responded with significantly enhanced DH reactions that could not be correlated with the cutaneous inoculation. Inoculation of the same number of dead organisms into infant mice neither primed animals for enhanced DH nor stimulated protective immunity. Antibody to cytoplasmic antigens ofCandidawas demonstrable in colonized animals, and its production was increased significantly in animals challenged iv with the highest dose of blastoconidia.