Effect of fructose on promoting fengycin biosynthesis in Bacillus amyloliquefaciens fmb-60
Effect of fructose on promoting fengycin biosynthesis in Bacillus amyloliquefaciens fmb-60
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DOI：
10.1111/jam.13291
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发表时间：
2016-12-01
影响因子：
4
通讯作者：
Lu, Z.
中科院分区：
文献类型：
--
作者：
Lu, H.;Qian, S.;Lu, Z.
目的：探讨碳水化合物对解淀粉芽孢杆菌FMB-60发酵产风霉素的影响。方法与结果：在发酵过程中添加果糖、阿拉伯糖和山梨醇，检测发酵产物和基因表达水平。与其他碳水化合物相比，果糖最有效地增加了细胞内合成风霉素所必需的氨基酸的浓度。在补料分批发酵条件下，风霉素的最大产量为392.87 mg L(-1)，提高了76%。此外，果糖还可通过增加枫霉素合成酶基因(fena、fenB、fenC、fend、fene和fente)和调控基因(comA、decu、decQ和sigma H)的表达来促进枫霉素的生物合成。结论：果糖对枫霉素的合成起重要作用，它可能通过提高枫霉素合成酶基因的表达和上调信号因子来促进枫霉素的生物合成。
Aims: To explore the influence of carbohydrates on the production of fengycin by Bacillus amyloliquefaciens fmb-60.Methods and Results: Fermentation and gene expression levels were examined following culture supplementation with fructose, arabinose and sorbitol. Compared with other carbohydrates, fructose most efficiently increased the concentration of the essential component amino acids for fengycin synthesis in the cell. The maximum production of fengycin was found to be 392.87 mg l(-1), increased by 76% in fed-batch fermentation. Furthermore, fructose enhanced the biosynthesis of fengycin by increasing the expression of fengycin synthetase genes (fenA, fenB, fenC, fenD, fenE and fenTE) and regulatory genes (comA, degU, degQ and sigma H).Conclusion: Fructose is important for fengycin production in B. amyloliquefaciens fmb-60, and it could enhance fengycin biosynthesis by raising the expression of the fengycin synthetase genes and upregulating signal factors.Significance and Impact of the Study: The results identify regulatory mechanisms involved in fengycin biosynthesis and provide an effective method to enhance production of fengycin.