TonB-dependent systems of uropathogenic Escherichia coli:: Aerobactin and heme transport and TonB are required for virulence in the mouse
TonB-dependent systems of uropathogenic Escherichia coli:: Aerobactin and heme transport and TonB are required for virulence in the mouse
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DOI：
10.1128/iai.69.10.6179-6185.2001
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发表时间：
2001-10-01
影响因子：
3.1
通讯作者：
Payne, SM
中科院分区：
文献类型：
--
作者：
Torres, AG;Redford, P;Payne, SM
尿路致病性大肠杆菌菌株CFT 073具有多种铁获取系统，包括血红素和铁载体转运蛋白。CFT 073的tonB突变体衍生物未能使用血红素作为铁源或利用铁载体肠杆菌素和需氧菌素，表明这些化合物在CFT 073中的转运是TonB依赖性的。TonB（-）衍生物在尿路感染的小鼠模型中显示出降低的毒力。当tonB基因被引入质粒时，毒力恢复。为了确定尿路感染期间个体TonB依赖性铁转运系统的重要性，构建了每种CFT 073高亲和力铁转运系统中的缺陷突变体，并在小鼠模型中进行了测试。小鼠毒力测定表明，在一个单一的铁转运系统缺陷的突变体能够感染肾脏接种时，作为一个纯文化，但不能有效地与野生型菌株在混合感染的竞争。这些结果表明TonB依赖系统在致尿路感染大肠杆菌的毒力中起作用。大肠杆菌菌株。
The uropathogenic Escherichia coli strain CFT073 has multiple iron acquisition systems, including heme and siderophore transporters. A tonB mutant derivative of CFT073 failed to use heme as an iron source or to utilize the siderophores enterobactin and aerobactin, indicating that transport of these compounds in CFT073 is TonB dependent. The TonB(-) derivative showed reduced virulence in a mouse model of urinary tract infection. Virulence was restored when the tonB gene was introduced on a plasmid. To determine the importance of the individual TonB-dependent iron transport systems during urinary tract infections, mutants defective in each of the CFT073 high-affinity iron transport systems were constructed and tested in the mouse model. Mouse virulence assays indicated that mutants defective in a single iron transport system were able to infect the kidney when inoculated as a pure culture but were unable to efficiently compete with the wild-type strain in mixed infections. These results indicate a role for TonB-dependent systems in the virulence of uropathogenic E. coli strains.