3D printing of hydroxyapatite/tricalcium phosphate scaffold with hierarchical porous structure for bone regeneration
3D printing of hydroxyapatite/tricalcium phosphate scaffold with hierarchical porous structure for bone regeneration
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DOI：
10.1007/s42242-019-00056-5
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发表时间：
2020-03-01
影响因子：
7.9
通讯作者：
Chen, Yong
中科院分区：
文献类型：
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作者：
Li, Xiangjia;Yuan, Yuan;Chen, Yong
近年来，三维（3D）打印支架引起了相当大的关注，因为它们为骨细胞组织再生提供了合适的环境，并且可以定制形状。在许多其他挑战中，材料组成和几何结构对支架的性能有重大影响。羟基磷灰石和磷酸三钙（HA/TCP）作为天然骨和牙齿的主要成分，具有良好的生物学性能，被广泛应用于骨支架的制备。已经研究了许多制造方法，试图获得具有微孔结构的HA/TCP支架，使细胞生长和营养物质转运。然而，目前的3D打印方法只能实现具有一定范围微孔结构的HA/TCP支架的制备。为了克服这一挑战，我们开发了一种基于浆料的微尺度掩模图像投影立体光刻，使我们能够形成具有复杂几何形状的HA/TCP基光固化悬浮液，包括仿生特征和分层孔隙率。本文研究了HA/TCP悬浮液的固化性能和物理性能，并开发了一种循环运动制备高粘度HA/TCP悬浮液的工艺。在此基础上，对支架材料的组成进行了优化。我们确定，具有仿生分级结构的30重量% HA/TCP支架具有上级的机械性能和孔隙率。在体外研究细胞增殖，并在具有颅神经嵴细胞和骨髓间充质干细胞的裸鼠体内长骨模型中进行手术。结果表明，我们的3D打印HA/TCP支架具有仿生分级结构，具有生物相容性，并具有足够的机械强度用于手术。
Three-dimensional (3D)-printed scaffolds have attracted considerable attention in recent years as they provide a suitable environment for bone cell tissue regeneration and can be customized in shape. Among many other challenges, the material composition and geometric structure have major impacts on the performance of scaffolds. Hydroxyapatite and tricalcium phosphate (HA/TCP), as the major constituents of natural bone and teeth, possess attractive biological properties and are widely used in bone scaffold fabrication. Many fabrication methods have been investigated in attempts to achieve HA/TCP scaffolds with microporous structure enabling cell growth and nutrient transport. However, current 3D printing methods can only achieve the fabrication of HA/TCP scaffolds with certain range of microporous structure. To overcome this challenge, we developed a slurry-based microscale mask image projection stereolithography, allowing us to form a HA/TCP-based photocurable suspension with complex geometry including biomimetic features and hierarchical porosity. Here, the curing performance and physical properties of the HA/TCP suspension were investigated, and a circular movement process for the fabrication of highly viscous HA/TCP suspension was developed. Based on these investigations, the scaffold composition was optimized. We determined that a 30 wt% HA/TCP scaffold with biomimetic hierarchical structure exhibited superior mechanical properties and porosity. Cell proliferation was investigated in vitro, and the surgery was conducted in a nude mouse in vivo model of long bone with cranial neural crest cells and bone marrow mesenchymal stem cells. The results showed our 3D-printed HA/TCP scaffold with biomimetic hierarchical structure is biocompatible and has sufficient mechanical strength for surgery.