The silkworm W chromosome is a source of female-enriched piRNAs
The silkworm W chromosome is a source of female-enriched piRNAs
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DOI：
10.1261/rna.027565.111
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发表时间：
2011-12-01
期刊：
影响因子：
4.5
通讯作者：
Katsuma, Susumu
中科院分区：
文献类型：
--
作者：
Kawaoka, Shinpei;Kadota, Koji;Katsuma, Susumu
在家蚕中，W染色体在雌性决定中起主导作用。然而，到目前为止，无论是蛋白质编码基因还是转录本都没有从W染色体中分离出来。相反，大量的功能性转座因子及其残余物积累在W染色体上。PIWI相互作用RNA（piRNA）是23-30 nt长的小RNA，其潜在地充当PIWI蛋白的序列特异性向导以沉默动物性腺中的转座子活性。在这项研究中，通过比较卵巢和睾丸来源的piRNA，我们鉴定了许多富含雌性的piRNA。我们的数据表明，雌性富集的piRNA来源于W染色体。此外，对来自一系列W染色体突变株的皮尔纳谱的比较分析揭示了一组特定的转座子衍生的piRNA在推定的性别决定区中的显著富集。总的来说，我们揭示了家蚕W染色体作为piRNA来源的性质。
In the silkworm, Bombyx mori, the W chromosome plays a dominant role in female determination. However, neither protein-coding genes nor transcripts have so far been isolated from the W chromosome. Instead, a large amount of functional transposable elements and their remnants are accumulated on the W chromosome. PIWI-interacting RNAs (piRNAs) are 23-30-nt-long small RNAs that potentially act as sequence-specific guides for PIWI proteins to silence transposon activity in animal gonads. In this study, by comparing ovary-and testis-derived piRNAs, we identified numerous female-enriched piRNAs. Our data indicated that female-enriched piRNAs are derived from the W chromosome. Moreover, comparative analyses on piRNA profiles from a series of W chromosome mutant strains revealed a striking enrichment of a specific set of transposon-derived piRNAs in the putative sex-determining region. Collectively, we revealed the nature of the silkworm W chromosome as a source of piRNAs.