Fructobacillus tropaeoli sp. nov., a fructophilic lactic acid bacterium isolated from a flower
Fructobacillus tropaeoli sp. nov., a fructophilic lactic acid bacterium isolated from a flower
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DOI：
10.1099/ijs.0.023838-0
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发表时间：
2011-04-01
影响因子：
2.8
通讯作者：
Dicks, Leon M. T.
中科院分区：
文献类型：
--
作者：
Endo, Akihito;Irisawa, Tomohiro;Dicks, Leon M. T.
从南非Tropaeolum majus的一朵花中分离到一株菌株F214-1(T)。基于16S rRNA基因序列的系统发育分析，该菌株与无花果芽孢杆菌和假无花果芽孢杆菌形成一个亚簇，基于recA基因序列的系统发育分析，该菌株与无花果芽孢杆菌形成一个亚簇。DNA-DNA杂交研究表明，菌株F214-1(T)在系统发育上与它的近亲不同。仅由d -葡萄糖、d -果糖和d -甘露醇发酵产生酸。d -果糖是唯一的碳源和能量来源，发酵速度比d -葡萄糖快。在厌氧条件下，菌株在d -葡萄糖上的生长非常弱，但氧和丙酮酸等外部电子受体促进了菌株在d -葡萄糖上的生长。d -葡萄糖产生等摩尔量的乳酸和乙酸。尽管该菌株具有专性异发酵代谢，但乙醇的产量非常低。基于这些数据，菌株F214-1(T)代表了果芽孢杆菌属的一种新的嗜果糖细菌，并被命名为tropaeoli sp. 11 .。型应变为F214-1(T) (=JCM 16675（T) =DSM 23246(T)）。
A fructophilic lactic acid bacterium, designated strain F214-1(T), was isolated from a flower of Tropaeolum majus in South Africa. Based on phylogenetic analysis of 16S rRNA gene sequences, the strain formed a subcluster with Fructobacillus ficulneus and Fructobacillus pseudo ficulneus and, based on recA gene sequences, the strain formed a subcluster with F ficulneus. DNA-DNA hybridization studies showed that strain F214-1(T) was phylogenetically distinct from its closest relatives. Acid was produced from the fermentation of D-glucose, D-fructose and D-mannitol only. D-Fructose was the preferred sole carbon and energy source and was fermented more rapidly than D-glucose. Growth of the strain on D-glucose under anaerobic conditions was very weak but external electron acceptors such as oxygen and pyruvate enhanced growth on D-glucose. Lactic acid and acetic acid were produced from D-glucose in equimolar amounts. Ethanol was produced at very low levels, despite the strain's obligately heterofermentative metabolism. Based on these data, strain F214-1(T) represents a novel species of fructophilic bacteria in the genus Fructobacillus, for which the name Fructobacillus tropaeoli sp. nov. is proposed. The type strain is F214-1(T) (=JCM 16675(T) =DSM 23246(T)).