Microcoil-based MRI: feasibility study and cell culture applications using a conventional animal system
Microcoil-based MRI: feasibility study and cell culture applications using a conventional animal system
复制标题
基于微线圈的 MRI：使用传统动物系统的可行性研究和细胞培养应用
DOI：
10.1007/s10334-011-0244-0
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
D. Elverfeldt
中科院分区：
文献类型：
--
作者：
H. Weber;N. Baxan;D. Paul;J. Maclaren;Daniel Schmidig;M. Mohammadzadeh;J. Hennig;D. Elverfeldt
ObjectThe本研究的目的是证明MR microimaging的可行性，在传统的9.4 T水平动物MRI系统使用商业可用的微线圈结合只有很小的修改系统，从而打开这个领域到一个更大的community.Materials和methodsCommercializially可用的RF微线圈设计的高分辨率NMR spectrometers被用于与定制的探头相结合。为此，在传输链中进行了更改，并对调整例程和图像采集序列进行了修改，所有这些都不需要昂贵的硬件。为了研究常规操作和高分辨率成像的可能性，分析了体模图像的质量。表面和螺线管微线圈的特点是他们的敏感体积和信噪比。此外，使用平面微线圈，以实现高分辨率图像的活胶质瘤细胞标记与MnCl2的可行性investigated.ResultsThe设置在这项工作中提出允许常规采集高品质的图像与高信噪比和各向同性分辨率高达10 μ mwithin an acceptable measurement time.ConclusionThis study demonstrates MR microscopy can be applied at low cost on animal MR imaging systems，其被广泛使用。活体胶质瘤细胞的成功成像表明，该技术有望成为生物医学研究中的一个有用工具。
ObjectThe aim of this study was to demonstrate the feasibility of MR microimaging on a conventional 9.4 T horizontal animal MRI system using commercial available microcoils in combination with only minor modifications to the system, thereby opening this field to a larger community.Materials and methodsCommercially available RF microcoils designed for high-resolution NMR spectrometers were used in combination with a custom-made probehead. For this purpose, changes within the transmit chain and modifications to the adjustment routines and image acquisition sequences were made, all without requiring expensive hardware. To investigate the extent to which routine operation and high-resolution imaging is possible, the quality of phantom images was analysed. Surface and solenoidal microcoils were characterized with regard to their sensitive volume and signal-to-noise ratio. In addition, the feasibility of using planar microcoils to achieve high-resolution images of living glioma cells labelled with MnCl2 was investigated.ResultsThe setup presented in this work allows routine acquisition of high-quality images with high SNR and isotropic resolutions up to 10 μm within an acceptable measurement time.ConclusionThis study demonstrates that MR microscopy can be applied at low cost on animal MR imaging systems, which are in widespread use. The successful imaging of living glioma cells indicates that the technique promises to be a useful tool in biomedical research.