Improvement of rice (Oryza sativa L.) seed oil quality through introduction of a soybean microsomal omega-3 fatty acid desaturase gene
Improvement of rice (Oryza sativa L.) seed oil quality through introduction of a soybean microsomal omega-3 fatty acid desaturase gene
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DOI：
10.1007/s00299-003-0609-6
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发表时间：
2003-06-01
影响因子：
6.2
通讯作者：
Takagi, Y
中科院分区：
文献类型：
--
作者：
Anai, T;Koga, M;Takagi, Y
微粒体ω-3脂肪酸去饱和酶是种子发育阶段产生n-3多不饱和脂肪酸α-亚麻酸的必需酶。我们构建了一个由玉米Ubi 1-P-int和大豆GmFAD 3 cDNA组成的嵌合基因，并通过农杆菌介导法将其导入水稻植株中。建立了10个含有嵌合基因的转化体，随后通过北方印迹证实了表达。此外，α-亚麻酸含量的T-1种子的显着增加高达10倍的对照，这种表型也稳定地遗传在T-2和T-3后代。这些结果表明，使用高活性启动子和GmFAD 3基因的组合可以容易地改变稻米种子油的α-亚麻酸含量。
Microsomal omega-3 fatty acid desaturase is an essential enzyme in the production of the n-3 polyunsaturated fatty acid alpha-linolenic acid during the seed developing stage. We have constructed a chimeric gene consisting of a maize Ubi1-P-int and a soybean GmFAD3 cDNA, which was introduced into rice plants by Agrobacterium-mediated transformation. Ten transformants containing the chimeric gene were established and expression subsequently confirmed by Northern blotting. Furthermore, alpha-linolenic acid content of the T-1 seeds increased dramatically up to tenfold that of the control, and this phenotype was also stably inherited in the T-2 and T-3 progenies. These results demonstrate that the alpha-linolenic acid content of rice seed oil can easily be altered using the combination of a high-activity promoter and a GmFAD3 gene.