Characterization of the functional gene and several processed pseudogenes in the human triosephosphate isomerase gene family
Characterization of the functional gene and several processed pseudogenes in the human triosephosphate isomerase gene family
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DOI：
10.1128/mcb.5.7.1694-1706.1985
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发表时间：
1985-07
影响因子：
5.3
通讯作者：
J. R. Brown;I. Daar;J. Krug;L. Maquat
中科院分区：
文献类型：
--
作者：
J. R. Brown;I. Daar;J. Krug;L. Maquat
从重组DNA文库中分离到人三磷酸酯异构酶的功能基因和3个无内含子假基因，并对其进行了详细的鉴定。该功能基因跨越3.5千碱基对，分为7个外显子。它的启动子含有假定的TATA和CCAAT盒子，并且极富含G和C残基（76%）。假基因与功能基因具有高度的同源性，但含有阻止合成活性三磷酸异构酶的突变。序列分化计算表明，这些假基因大约出现在1800万年前。我们提出的证据表明，有一个单一的功能基因在人类三磷酸异构酶基因家族。
The functional gene and three intronless pseudogenes for human triosephosphate isomerase were isolated from a recombinant DNA library and characterized in detail. The functional gene spans 3.5 kilobase pairs and is split into seven exons. Its promoter contains putative TATA and CCAAT boxes and is extremely rich in G and C residues (76%). The pseudogenes share a high degree of homology with the functional gene but contain mutations that preclude the synthesis of an active triosephosphate isomerase enzyme. Sequence divergence calculations indicate that these pseudogenes arose approximately 18 million years ago. We present evidence that there is a single functional gene in the human triosephosphate isomerase gene family.