Cloning and structural analysis of the cDNA and the gene encoding the murine type 1 sigma receptor
Cloning and structural analysis of the cDNA and the gene encoding the murine type 1 sigma receptor
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DOI：
10.1006/bbrc.1997.7840
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发表时间：
1997-12-18
影响因子：
3.1
通讯作者：
Ganapathy, V
中科院分区：
文献类型：
--
作者：
Seth, P;Leibach, FH;Ganapathy, V
我们已经分离出一种编码功能性1型西格玛受体的小鼠cDNA。通过在哺乳动物细胞中表达该cDNA，并测定其与氟哌啶醇的结合情况，确定了该cDNA的功能特性，以该cDNA为探针，分离出完整的1型sigma受体基因的小鼠基因组克隆，对该基因进行了完整的测序，推断了其外显子-内含子结构，分析了转录因子结合位点的启动子区序列。该基因（类似于7 kbp）不含tata，但在转录起始位点上游含有CCAATC和GC盒，该基因由4个外显子和3个内含子组成，5'侧区包含AP-1， AP-2， GATA-1和类固醇受体的推定结合位点，(C) 1997学术出版社。
We have isolated a mouse cDNA which codes for a functional type 1 sigma receptor. The functional identity of the cDNA was established by expressing the cDNA in mammalian cells and measuring the cDNA-induced binding of haloperidol, Using this cDNA as the probe, we have isolated a murine genomic clone which contains the type 1 sigma receptor gene in its entirety, We have sequenced the gene completely, deduced the exon-intron organization and analyzed the promoter region sequence for transcription factor binding sites. The gene (similar to 7 kbp) is TATA-less but contains CCAATC and GC boxes immediately upstream of the transcription start site, The gene consists of 4 exons and 3 introns, The 5'-flanking region contains putative binding sites for AP-1, AP-2, GATA-1 and steroid receptors, (C) 1997 Academic Press.