Microbial community analysis in the rhizosphere of a transgenic tomato that overexpresses 3-hydroxy-3-methylglutaryl coenzyme A reductase.
Microbial community analysis in the rhizosphere of a transgenic tomato that overexpresses 3-hydroxy-3-methylglutaryl coenzyme A reductase.
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DOI：
10.1264/jsme2.21.261
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发表时间：
2006-01-01
影响因子：
2.2
通讯作者：
Fujimura, T.
中科院分区：
文献类型：
--
作者：
Ikeda, S.;Omura, T.;Fujimura, T.
利用核糖体基因间间隔区分析（RISA）和细菌几丁质酶和nifH基因的末端限制性片段长度多态性（T-RFLP）分析，研究了3-羟基-3-甲基戊二酰辅酶A还原酶基因过表达对转基因番茄根际微生物多样性的影响。虽然RISA和T-RFLP的整体轮廓显示转基因番茄和非转基因番茄之间的高度相似性，几个多态性DNA检测到的定性和定量评价的差异带。序列分析表明，差异带克隆在RISA显示低水平的相似性，已知的物种，但最有可能来自未培养的微生物。对几丁质酶和nifH基因的T-RFLP分析中克隆到的两条差异带进行系统发育分析，表明这两条差异带分别属于类链霉菌几丁质酶组和蓝藻nifH组。
The impact of overexpression of the 3-hydroxy-3-methylglutaryl coenzyme A reductase gene on microbial diversity in the rhizosphere of a transgenic tomato was examined using a ribosomal intergenic spacer analysis (RISA) and a terminal restriction fragment length polymorphism (T-RFLP) analysis of two functional bacterial genes (bacterial chitinase and nifH genes). While the overall profiles of both RISA and T-RFLP revealed high similarity between the transgenic and non-trangenic tomato, several polymorphic DNAs were detected as differential bands in both the qualitative and quantitative evaluations. Sequence analysis suggested that differential bands cloned in RISA showed low levels of similarity to known species but were most likely derived from uncultured microbes. The phylogenetic analyses of two differential bands cloned in the T-RFLP analysis of the chitinase and nifH genes indicated that these bands belonged to Streptomyces like chitinase group and cyanobacterial nifH group, respectively.