Morphological change and characteristics of myofibroblasts during the growth process of benign prostatic hyperplasia
Morphological change and characteristics of myofibroblasts during the growth process of benign prostatic hyperplasia
复制标题
良性前列腺增生生长过程中肌成纤维细胞的形态变化及特征
作者：
Hata J;Tanji R;Onagi A;Honda-Takinami R;Matsuoka K;Hoshi S;Sato Y;Akaihata H;Haga N;Kojima Y.
目的探讨肌成纤维细胞在良性前列腺增生（BPH）生长过程中的形态学变化及特征。方法采用基质成分主导型良性前列腺增生（BPH）大鼠模型，观察肌成纤维细胞在前列腺生长过程中的特征。通过蛋白质印迹法评价转化生长因子β1和胰岛素样生长因子结合蛋白3表达（n= 6）。我们使用双重免疫组织化学染色来评估良性前列腺增生组织中α-平滑肌肌动蛋白和波形蛋白阳性的肌成纤维细胞的数量。在大鼠泌尿生殖窦植入后2、3和8周，对良性前列腺增生的表达和组织学分析（n= 6）。结果前列腺增生组织中转化生长因子β1和胰岛素样生长因子结合蛋白3的表达均显著上调（P< 0.05）。良性前列腺增生组织中肌成纤维细胞的数量明显多于正常前列腺组织（P< 0.05），且大量分布于间质区。转化生长因子β1和胰岛素样生长因子结合蛋白3的表达和肌成纤维细胞的数量呈时间依赖性增加（P< 0.05），生长因子的表达先于肌成纤维细胞的增加。电镜证实，在人良性前列腺增生组织中，肌成纤维细胞祖细胞主要分布于纤维区周围，具有丰富的应力纤维。结论在良性前列腺增生组织的生长过程中，转化生长因子β1和胰岛素样生长因子结合蛋白3诱导其向肌成纤维细胞分化。肌成纤维细胞祖细胞似乎与人良性前列腺增生的前列腺纤维化有关。
ObjectivesTo clarify the morphological change and characteristics of myofibroblast during the growth process of benign prostatic hyperplasia.MethodsThis study examined the characteristics of myofibroblasts during the growth process of the prostate in the stromal component‐dominant benign prostatic hyperplasia rat model. Transforming growth factor‐β1 and insulin‐like growth factor‐binding protein 3 expression were evaluated by western blotting (n= 6). We used double immunohistochemical staining to evaluate the number of myofibroblasts positive for α‐smooth muscle actin and vimentin in benign prostatic hyperplasia tissues. Expression and histological analyses of the benign prostatic hyperplasia were also carried out in rats at 2, 3 and 8 weeks after urogenital sinus implantation (n= 6). To evaluate the fine morphological characteristics of myofibroblasts in human benign prostatic hyperplasia tissues, electron microscopy analysis was additionally carried out.ResultsThere was a significant upregulation of the transforming growth factor‐β1 and insulin‐like growth factor‐binding protein 3 expression in benign prostatic hyperplasia (P< 0.05). There was a significant increase in the number of myofibroblasts in benign prostatic hyperplasia (P< 0.05) compared with normal prostate, with these abundantly located in the stromal area. The transforming growth factor‐β1 and insulin‐like growth factor‐binding protein 3 expression and number of myofibroblasts showed a time‐dependent increase (P< 0.05), with growth factor expressions preceding the myofibroblast increase. Electron microscopy confirmed that the myofibroblast progenitor cells, which possess abundant stress fibers, were predominantly located around fibrous areas in human benign prostatic hyperplasia.ConclusionsDifferentiation into myofibroblasts induced by transforming growth factor‐β1 and insulin‐like growth factor‐binding protein 3 actively occurs during the growth process of benign prostatic hyperplasia. Myofibroblast progenitor cells seem to be associated with prostatic fibrosis in human benign prostatic hyperplasia.