Serological biomarkers for the diagnosis of Mycobacterium abscessus infections in cystic fibrosis patients
Serological biomarkers for the diagnosis of Mycobacterium abscessus infections in cystic fibrosis patients
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DOI：
10.1016/j.jcf.2021.08.019
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发表时间：
2022-03-22
影响因子：
5.2
通讯作者：
Herrmann, Jean-Louis
中科院分区：
文献类型：
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作者：
Le Moigne, Vincent;Roux, Anne-Laure;Herrmann, Jean-Louis
背景：培养条件有时使检测非结核分枝杆菌(NTM)变得困难，尤其是脓肿分枝杆菌，一种新出现的囊性纤维化(CF)病原体。方法：作为诊断准确性研究的一部分，共有173例血清CF型患者进行了诊断，其中包括33例Mc阳性培养的患者和31例非CF型健康对照(HC)。分别使用4种脓肿分枝杆菌抗原，包括两种表面提取物(间期(INP)和一种TLR2阳性提取物(TLR2eF))和两种重组蛋白(rMAB_2545c和rMAB_0555，也称为磷脂酶C(RPLC))。TLR2eF和RPLC的敏感性分别为81.2%(95%CI：65.7-92.3%)和87.9%(95%CI：71.9-95.6%)，特异性分别为88.9%(95%CI：85.3-94.8%)和84.8%(95%CI：80.6-91.5%)。当考虑所有血清均为阳性，两种试验中至少有一种为阳性，而两种试验均为阴性时，我们获得了93.9%的敏感性和80.7%的特异性。结论：在脓肿培养阳性的CF患者中，获得了高滴度的TLR2eF和RPLC抗体，这使得我们可以将这些血清学标志物作为检测感染脓肿分支杆菌的CF患者的潜在工具。(C)2021年欧洲囊性纤维化学会。爱思唯尔出版，版权所有。
Background: Culture conditions sometimes make it difficult to detect non-tuberculous mycobacteria (NTM), particularly Mycobacterium abscessus, an emerging cystic fibrosis (CF) pathogen. The diagnosis of NTM positive cases not detected by classical culture methods might benefit from the development of a serological assay.Methods: As part of a diagnostic accuracy study, a total of 173 sera CF-patients, including 33 patients with M. abscessus positive cultures, and 31 non-CF healthy controls (HC) were evaluated. Four M. abscessus antigens were used separately, comprising two surface extracts (Interphase (INP) and a TLR2 positive extract (TLR2eF)) and two recombinant proteins (rMAB_2545c and rMAB_0555 also known as the phospholipase C (rPLC)).Results: TLR2eF and rPLC were the most efficient antigens to discriminate NTM-culture positive CFpatients from NTM-culture negative CF-patients. The best clinical values were obtained for the detection of M. abscessus-culture positive CF-patients; with sensitivities for the TLR2eF and rPLC of 81.2% (95% CI:65.7-92.3%) and 87.9% (95% CI:71.9-95.6%) respectively, and specificities of 88.9% (95% CI:85.3-94.8%) and 84.8% (95% CI:80.6-91.5%) respectively. When considering as positive all sera, giving a positive response in at least one of the two tests, and, as negative, all sera negative for both tests, we obtained a sensitivity of 93.9% and a specificity of 80.7% for the detection of M. abscessus-culture positive CF-patients.Conclusion: High antibody titers against TLR2eF and rPLC were obtained in M. abscessus-culture positive CF-patients, allowing us to consider these serological markers as potential tools in the detection of CFpatients infected with M. abscessus. (C) 2021 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.