Effect of TongXie-YaoFang on Cl(-) and HCO3 (-) Transport in Diarrhea-Predominant Irritable Bowel Syndrome Rats.
Effect of TongXie-YaoFang on Cl(-) and HCO3 (-) Transport in Diarrhea-Predominant Irritable Bowel Syndrome Rats.
复制标题
痛泻药方对腹泻型肠易激综合征大鼠Cl-和HCO3-转运的影响
DOI：
10.1155/2016/7954982
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Xie J
中科院分区：
文献类型：
--
作者：
Lu X;Zhang S;Yang C;Wang Z;Zhao L;Wu Z;Xie J
痛泻要方能有效缓解腹泻型肠易激综合征（D-IBS）患者的症状。然而，治疗机制尚未完全阐明。本研究旨在观察通心泄肺方对5-羟色胺（5-HT）诱导的D-IBS大鼠结肠离子转运的影响。采用新生儿母亲分离（NMS）结合束缚应激（RS）的方法建立D-IBS模型。将模型大鼠随机分为NMS + RS组和TXYF方组，正常对照组（不处理）为NH组。NMS + RS组5-HT引起的短路电流变化（Δ Isc）低于NH组和TXYF组。去除细胞外Cl −或HCO3 −或基底侧Na+或分别阻断囊性纤维化跨膜传导调节因子（CFTR）、Na +-K +-2Cl −协同转运蛋白（NKCC）、Na +-HCO3 −协同转运蛋白、Cl −/HCO3 −交换蛋白、K+通道或Na +/K +-ATP酶后，3组间5-HT诱导的Δ I sc无差异。这些数据表明，TXYF可以调节5-HT诱导的Cl −和HCO3 −分泌，可能通过CFTR，NKCC，Na +-HCO3 −协同转运蛋白，Cl −/HCO3 −交换器，K+通道和Na +/K +-ATP酶的联合作用介导。
TongXie-YaoFang (TXYF) can effectively alleviate the symptoms of diarrhea-predominant irritable bowel syndrome (D-IBS) patients. However, the curative mechanism has not been fully clarified. The study was designed to investigate the effect of TXYF on the colonic ion transport induced by serotonin (5-HT) in D-IBS rats. A method of multiple stress (neonatal maternal separation (NMS) combined with restraint stress (RS)) was used to induce the D-IBS model. The model rats were randomly divided into two groups: NMS + RS group and TXYF-formula group, and the normal control (no handling) rats were classified as NH group. In the NMS + RS group, the change of short-circuit current (ΔI sc) induced by 5-HT was lower than that in the NH and TXYF-formula groups. After removing of the extracellular Cl− or HCO3 − or basolateral Na+ or blocking the cystic fibrosis transmembrane conductance regulator (CFTR), Na+-K+-2Cl− cotransporter (NKCC), Na+-HCO3 − cotransporter, Cl−/HCO3 − exchanger, K+ channel, or Na+/K+-ATPase, respectively, there was no difference in 5-HT-induced ΔI sc among the three groups. These data suggest that TXYF can regulate 5-HT-induced Cl− and HCO3 − secretion, possibly mediated by the combined action of CFTR, NKCC, Na+-HCO3 − cotransporter, Cl−/HCO3 − exchanger, K+ channel, and Na+/K+-ATPase.