INTERSTITIAL PNEUMONITIS AND LYMPHOCYTIC BRONCHIOLITIS/BRONCHITIS AS A DIRECT RESULT OF ACUTE LETHAL GRAFT‐VERSUS-HOST DISEASE DUPLICATE THE HISTOPATHOLOGY OF LUNG ALLOGRAFT REJECTION1
INTERSTITIAL PNEUMONITIS AND LYMPHOCYTIC BRONCHIOLITIS/BRONCHITIS AS A DIRECT RESULT OF ACUTE LETHAL GRAFT‐VERSUS-HOST DISEASE DUPLICATE THE HISTOPATHOLOGY OF LUNG ALLOGRAFT REJECTION1
复制标题
急性致死性移植物抗宿主病直接导致的间质性肺炎和淋巴细胞性细支气管炎/支气管炎与肺同种异体移植物排斥的组织病理学重复1
DOI：
10.1097/00007890-199405820-00013
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发表时间：
1994
期刊：
影响因子：
6.2
通讯作者：
J. Clancy
中科院分区：
文献类型：
--
作者：
Diane L. Workman;J. Clancy
肺部并发症通常是急性移植物抗宿主病（GVHD）的致命部分。尽管间质性肺炎和淋巴细胞性支气管炎被认为是急性 GVHD 的组成部分，但之前的研究尚未确定这些组织病理学的发生或其对可能由急性 GVHD 直接导致的肺部综合征的影响之间的相关性。本研究使用未接受化疗、免疫抑制药物或明显感染的成年未辐射 (DA x LEW) F1 杂交大鼠来研究急性 GVHD 期间肺部病理学的这些方面。 F1 动物静脉注射 1 x 106 DA 亲本淋巴细胞/克体重，到第 21 天时发病率和死亡率为 100%。同基因注射或非注射的 F1 对照动物均不包含任何可观察或可测量的组织病理学。此外，根据特定组织和免疫组织化学染色确定，GVHD 和对照组织不含有细菌、真菌或 CMV 污染。 GVHD 动物在注射后第 3、7、10、14 和 15-21 天被处死。全叶组织切片 (4 μU) 用 H&E 染色，并使用光学显微镜图像分析对预定组织部位内的组织学改变进行量化。肺泡间隔宽度和血管周围浸润体积密度在第 7 天时显着高于对照组，并在第 21 天分别增加了 2.4 倍和 2.6 倍。这些数据证实了间质性肺炎和淋巴细胞性细支气管炎/支气管炎的发展，其与肺同种异体移植排斥的组织病理学重复。在成年 F1 大鼠急性 GVHD 期间发现与肺同种异体移植排斥相对应的肺部病理学，表明肺是潜在的靶器官。
Pulmonary complications are often lethal components of acute graft-vs.-host disease (GVHD). Although interstitial pneumonitis and lymphocytic bronchitis have been implicated as elements of acute GVHD, previous studies have not determined a correlation between the onset of these histopathologies or their contribution to a pulmonary syndrome that may occur as a direct result of acute GVHD. The present study used the adult, nonirradiated (DA x LEW) F1 hybrid rat in the absence of chemotherapy, immunosuppressive drugs, or overt infection to study these aspects of pulmonary pathology during acute GVHD. F1 animals were intravenously injected with 1 x 106 DA parental lymphoid cells/g body weight, which produced 100% morbidity and mortality by day 21. Neither syngeneically injected nor noninjected F1 control animals contained any observable or measurable histopathology. In addition, GVHD and control tissues did not contain bacterial, fungal, or CMV contamination as determined by specific tissue and immunohistochemical staining. GVHD animals were killed on days 3, 7,10,14, and 15–21 following injection. Whole-lobe tissue sections (4 μU) were stained with H&E, and histologic alterations within predetermined tissue sites were quantified using light-microscopic image analysis. Alveolar septal widths and perivascular infiltrate volume densities were increased significantly above con- trols by day 7, and reached 2.4− and 2.6-fold increases, respectively, by day 21. These data corroborated the development of an interstitial pneumonitis and lymphocytic bronchiolitis/bronchitis that duplicated the histopathology of lung allograft rejection. The discovery of pulmonary pathology corresponding to lung allograft rejection during acute GVHD in the adult F1 rat implicates the lung as a potential target organ.