In vitro engineering of human ear-shaped cartilage assisted with CAD/CAM technology
In vitro engineering of human ear-shaped cartilage assisted with CAD/CAM technology
复制标题
CAD/CAM技术辅助的人耳形软骨体外工程
DOI：
10.1016/j.biomaterials.2009.11.080
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发表时间：
2010-03-01
期刊：
影响因子：
14
通讯作者：
Cao, Yilin
中科院分区：
文献类型：
--
作者：
Liu, Yu;Zhang, Lu;Cao, Yilin
由于缺乏合适的支架，具有复杂结构的软骨组织（如人耳）的体外工程仍然是一个巨大的挑战。虽然聚乙醇酸（PGA）已成为最成功的软骨再生支架之一，如何克服其局限性，实现理想的机械强度和精确的形状控制仍然是一个未解决的问题。在这项研究中，PGA支架的机械强度提高与聚乳酸（PLA）涂层。通过平衡支架的生物相容性和机械强度，优化PLA的含量。根据正常人耳CT扫描数据，采用计算机辅助设计与制造（CAD/CAM）技术制作耳模，将PLA/PGA支架压入耳模中，制成与正常人耳镜像对称的人耳模型。通过使用3D激光扫描系统的形状分析，与阳性耳模相比，耳形支架达到了超过97%的相似性水平。最重要的是，在软骨细胞接种后，构建体在培养期间基本上保持了原始形状，相似性水平超过84%。12周时，构建体形成耳状软骨样组织，其显示具有丰富的软骨细胞外基质和成熟陷窝的组织结构。此外，12周时的耳状软骨也表现出良好的弹性和良好的机械强度。这些结果可能为通过体外工程方法重建具有复杂形状的软骨组织（如人耳）提供有用的策略。(C)2009爱思唯尔有限公司保留所有权利。
Due to the lack of appropriate scaffolds, the in vitro engineering of cartilage tissue with a sophisticated structure, such as a human ear, remains a great challenge. Although polyglycolic acid (PGA) has become one of the most successful scaffolds for cartilage regeneration, how to overcome its limitations in achieving desirable mechanical strength and accurate control over shape remains an unsolved problem. In this study, the mechanical strength of PGA scaffold was enhanced by coating with polylactic acid (PLA). The content of PLA was optimized by balancing the scaffold's biocompatibility and mechanical strength. The PLA/PGA scaffold was then fabricated into a human ear-shape mirror-symmetrical to a normal ear by pressing the scaffold in the ear negative molds, which were fabricated by the computer aided design and manufacturing (CAD/CAM) technique according to the CT scan data from the normal ear. The ear-shaped scaffold reached a similarity level of over 97% compared to the positive ear mold by the shape analysis using a 3D laser scan system. Most importantly, after chondrocyte seeding, the constructs largely retained the original shape during culture with a similarity level of over 84%. Furthermore, the constructs formed ear-shaped cartilage-like tissues at 12 weeks, which revealed a tissue structure with abundant cartilage extracellular matrices and mature lacuna. Additionally, the ear-shaped cartilage at 12 weeks also exhibited fine elasticity and good mechanical strength. These results may provide a useful strategy for reconstructing cartilage tissue with complicated shapes such as a human ear by an in vitro engineering approach. (C) 2009 Elsevier Ltd. All rights reserved.