Production of a monoclonal antibody to canine thymus and activation-regulated chemokine (TARC) and detection of TARC in lesional skin from dogs with atopic dermatitis
Production of a monoclonal antibody to canine thymus and activation-regulated chemokine (TARC) and detection of TARC in lesional skin from dogs with atopic dermatitis
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DOI：
10.1016/j.vetimm.2004.08.021
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发表时间：
2005-01-10
影响因子：
1.8
通讯作者：
Iwabuchi, S
中科院分区：
文献类型：
--
作者：
Maeda, S;Tsukui, T;Iwabuchi, S
为研究TARC与犬特应性皮炎（AD）免疫发病机制的关系，研制了抗犬胸腺活化调节趋化因子（TARC/CCL 17）的单克隆抗体。重组犬TARC用E. coli表达系统。transwell趋化实验结果表明，重组犬TARC对表达CC趋化因子受体4（CCR 4）的犬淋巴细胞具有趋化活性。然后用重组犬TARC免疫小鼠以获得单克隆抗体。在由此获得的单克隆抗体中，发现一种单克隆抗体（CTA-1）在ELISA和流式细胞术测定中分别与重组和真实犬TARC反应。单克隆抗体CTA-1的免疫组化分析表明，角质形成细胞是主要的TARC产生细胞在病变的皮肤与AD的狗。(C)2004 Elsevier B. V.保留所有权利。
A monoclonal antibody to canine thymus and activation-regulated chemokine (TARC/CCL17) was developed to examine the association of TARC with the immunopathogenesis of canine atopic dermatitis (AD). Recombinant canine TARC was prepared using an E. coli expression system. Results of transwell chemotaxis assay demonstrated that the recombinant canine TARC showed chemotactic activity for canine lymphoid cells expressing CC chemokine receptor 4 (CCR4). Mice were then immunized with the recombinant canine TARC to obtain monoclonal antibodies. Among the monoclonal antibodies thereby obtained, one monoclonal antibody (CTA-1) was found to react with both recombinant and authentic canine TARC in ELISA and flowcytometric assays, respectively. Immunohistochemical analysis using the monoclonal antibody CTA-1 demonstrated that keratinocytes were major TARC producing cells in lesional skin of dogs with AD. (C) 2004 Elsevier B.V. All rights reserved.