Doxycycline-functionalized polymeric nanoparticles inhibit Enterococcus faecalis biofilm formation on dentine
Doxycycline-functionalized polymeric nanoparticles inhibit Enterococcus faecalis biofilm formation on dentine
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DOI：
10.1111/iej.13436
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发表时间：
2020-11-22
影响因子：
5
通讯作者：
Osorio, R.
中科院分区：
文献类型：
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作者：
Arias-Moliz, M. T.;Baca, P.;Osorio, R.
目的在实验室环境中评价新型矿化聚合物纳米颗粒（NP）在应用于牙本质表面时的抗微生物特性和抑制生物膜形成的潜力，并确定这些NP与锌的功能化的效果，方法在感染肠球菌的人牙本质块上分析聚合物NP的抗微生物活性和生物膜形成的抑制在应用NP之前或之后的粪便。采用共聚焦激光扫描显微镜下的LIVE/DEAD（R）测试和细菌培养来分析生物膜生物体积和细菌活力。还采用场发射扫描电子显微镜来评估生物膜形态。采用Welch校正的单因素方差分析和Games-Howell检验进行组间比较。粪便生物膜，因为它们提供最低的生物体积（3865.7 ± 2926.97 μ m（3）; P < 0.001）和最高的死亡/损伤细胞百分比（79.93 +/- 18.40%; P < 0.001），其次是Dox-NPs（生物体积：19，041.55 +/-17，638.23 μ m（3），死亡/损伤细胞：45.53 +/- 26.50%; P < 0.001）。负载多西环素的纳米颗粒具有最大的生物膜形成抑制值，具有最低的生物膜生物体积（8517.65 +/- 7055.81 μ m（3）; P < 0.001）和高的死亡/损伤细菌百分比（68.68 +/- 12.50%; P < 0.001）。未官能化的NP不降低生物质生长（P > 0.05），但获得了最大百分比的受损细胞（93 +/-8.23%; P < 0.001），能够破坏生物膜形成。它也产生了牙本质小管闭塞，潜在的干扰细菌tubule penetration.Conclusions新一代的生物活性纳米填料（多西环素功能化的聚合物纳米粒子）的抗菌活性和闭塞的牙本质小管。将这些纳米颗粒制成根管封闭剂可能有可能提高根管治疗的效果。
Aim To evaluate in a laboratory setting the antimicrobial properties and the potential to inhibit biofilm formation of novel remineralizing polymeric nanoparticles (NPs) when applied to dentine surfaces and to ascertain the effect of the functionalization of these NPs with zinc, calcium or doxycycline.Methodology The antimicrobial activity and inhibition of biofilm formation of polymeric NPs were analysed on human dentine blocks that were infected with Enterococcus faecalis before or after application of NPs. LIVE/DEAD (R) testing under Confocal Laser Scanning Microscopy and bacterial culturing were employed to analyse biofilm biovolume and bacterial viability. Field Emission Scanning Electron Microscopy was also employed to assess biofilm morphology. One-way anova with Welch's correction and post hoc comparison by the Games-Howell test were performed for comparisons between groups.Results The un-functionalized NPs displayed the greatest antimicrobial activity against E. faecalis biofilms as they provided the lowest biovolume (3865.7 +/- 2926.97 mu m(3); P < 0.001) and the highest dead/injured cells percentage (79.93 +/- 18.40%; P < 0.001), followed by Dox-NPs (biovolume: 19,041.55 +/- 17,638.23 mu m(3), dead/injured cells: 45.53 +/- 26.50%; P < 0.001). Doxycycline-loaded NPs had the largest values of inhibition of biofilm formation with the lowest biofilm biovolume (8517.65 +/- 7055.81 mu m(3); P < 0.001) and a high dead/injured bacterial percentage (68.68 +/- 12.50%; P < 0.001). Un-functionalized NPs did not reduce biomass growth (P > 0.05), but attained the largest percentage of compromised cells (93 +/- 8.23%; P < 0.001), being able to disrupt biofilm formation. It also produced occlusion of dentinal tubules, potentially interfering with bacterial tubule penetration.Conclusions A new generation of bioactive nano-fillers (doxycycline-functionalized polymeric NPs) had antibacterial activity and occluded dentinal tubules. Incorporating these NPs into endodontic sealers may have the potential to enhance the outcome of root canal treatment.