Chemical composition and immunobiological properties of lipopolysaccharide and lipid-associated proteoglycan from Actinobacillus actinomycetemcomitans.
Chemical composition and immunobiological properties of lipopolysaccharide and lipid-associated proteoglycan from Actinobacillus actinomycetemcomitans.
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来自放线放线杆菌的脂多糖和脂质相关蛋白聚糖的化学成分和免疫生物学特性。
DOI：
10.1111/j.1600-0765.1986.tb01488.x
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发表时间：
1986
影响因子：
3.5
通讯作者：
S. Hamada
中科院分区：
文献类型：
--
作者：
T. Nishihara;T. Fujiwara;T. Koga;S. Hamada
用苯酚-水法和丁醇-水法从伴放线放线杆菌29523菌株中分离出脂多糖和脂相关蛋白多糖。内毒素由41%的中性糖组成。氨基己糖8%，脂肪酸31%，蛋白质2%，磷2%，丁醇水提液化气由中性糖13%，氨基己糖2%，脂肪酸14%组成。56%的蛋白质和1%的磷。脂多糖和液化石油气的主要脂肪酸为β-羟基麦芽酸、肉豆蔻酸和棕榈酸。这些制剂可诱导内毒素应答的C3H/HEN小鼠脾细胞的有丝分裂反应和多克隆反应，并增强脾细胞对绵羊红细胞的体外免疫应答。对肠杆菌科细菌脂多糖无反应的C3H/HeJ小鼠脾细胞对丁醇水提液有反应，而对苯酚水提液无反应。伴放线放线杆菌脂多糖具有与大肠埃希菌K235脂多糖相当的凝血活性。这些结果表明，伴生放线菌脂多糖在生物学和免疫化学上属于肠杆菌科类型。
Lipopolysaccharide (LPS) and lipid-associated proteoglycan (LPG) were isolated from Actinobacillus actinomycetemcomitans ATCC 29523 by the phenol-water and butanol-water procedures. The LPS was composed of 41 % neutral sugar. 8% hexosamine, 31 % fatty acid, 2% protein, and 2% phosphorus, while the butanol-water-extracted LPG was composed of 13% neutral sugar, 2% hexosamine, 14% fatty acid. 56% protein, and 1% phosphorus. The major fatty acids of LPS and LPG were β-hydroxymyristic, myristic, and palmitic acids. These preparations induced mitogenic and polyclonal responses of LPS-responsive C3H/HeN mouse spleen cells and enhanced in vitro immune responses of the spleen cells to sheep erythrocytes. Spleen cells of C3H/HeJ mouse that were nonresponsive to enterobacteriaceal LPS responded to the butanol-water-extracted LPG, but not to the phenol-water-extracted LPS. The Limulus amebocyte lysate clotting activity of A. actinomycetemcomitans LPS was comparable to that of Escherichia coli K235 LPS. These results indicate that A. actinomycetemcomitans LPS is biologically and immunochemically of Enterobacteriaceae type.
伴放线放线杆菌菌株 Y4 脂多糖对小鼠腹腔巨噬细胞的体外影响。
影响因子：
2.8
作者：
Barker,KA;Holt,SC
通讯作者：
Holt,SC