Determination of reference ranges for immature platelet and reticulocyte fractions and reticulocyte hemoglobin equivalent.
Determination of reference ranges for immature platelet and reticulocyte fractions and reticulocyte hemoglobin equivalent.
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DOI：
10.1016/j.bjhh.2016.07.001
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发表时间：
2016-10
影响因子：
--
通讯作者：
Scotti L
中科院分区：
文献类型：
--
作者：
Morkis IV;Farias MG;Scotti L
未成熟血小板和未成熟网织红细胞部分代表最近释放到循环中并因此具有较高RNA含量的血小板和网织红细胞的比率。它们被认为是骨髓恢复的早期指标。本研究的目的是确定参考范围的未成熟血小板和网织红细胞分数的血液学正常的个人在一所大学医院。使用Sysmex XE-5000™分析仪分析在乙二胺四乙酸K3中采集的静脉血样。纳入血小板和网织红细胞计数在参考范围内，血细胞计数在实验室筛选标准内的个体。排除了具有可能影响血液学结果的临床状况的个体。评价未成熟血小板分数、高、中、低荧光网织红细胞分数和网织红细胞血红蛋白当量。根据国际临床化学联合会的建议确定参考范围。对132例门诊患者进行了评价。平均年龄为44岁（范围：13-80岁），72例（54.5%）为在大学医院接受治疗的女性。平均血小板计数为250.8 × 109/L，平均网织红细胞计数为0.052 × 109/L。获得以下参考范围：未成熟网织红细胞分数1.6- 12.1%，高、中、低荧光网织红细胞分数分别为0.0- 1.7%、1.6-11.0%和87.9- 98.4%，网织红细胞血红蛋白当量为30.0-37.6%，未成熟血小板分数为0.8- 5.6%。性别间未成熟血小板分数存在统计学显著差异（p值= 0.006），女性为0.8-4.7%，男性为0.7-6.1%。未成熟网织红细胞分数与网织红细胞计数直接相关。确定参考范围对于引入新参数至关重要。本文获得的参考范围证实了先前出版物中报道的参考范围，并将有助于指数的临床和实验室应用。
The immature platelet and immature reticulocyte fractions represent the ratios of platelets and reticulocytes recently released into the circulation and thus with higher RNA content. They are considered early indicators of bone marrow recovery. The aim of this study was to determine the reference ranges for the immature platelet and reticulocyte fractions of hematologically normal individuals in a university hospital. Venous blood samples collected in ethylenediaminetetraacetic acid K3 were analyzed using a Sysmex XE-5000™ analyzer. Individuals with platelet and reticulocyte counts within the reference ranges, and a blood count within the laboratory's screening criteria were included. Individuals with clinical conditions that could affect hematological results were excluded. The immature platelet fraction, high, medium and low fluorescence reticulocyte fractions and reticulocyte hemoglobin equivalent were evaluated. The reference ranges were determined according to the recommendations of the International Federation of Clinical Chemistry. One hundred and thirty-two outpatients were evaluated. The mean age was 44 years (range: 13–80 years), 72 (54.5%) were women treated in a university hospital. The mean platelet count was 250.8 × 109/L and the mean reticulocyte count was 0.052 × 109/L. The following reference ranges were obtained: immature reticulocyte fraction 1.6–12.1%, the high, medium and low fluorescence reticulocyte fractions were 0.0–1.7%, 1.6–11.0% and 87.9–98.4%, respectively, the reticulocyte hemoglobin equivalent was 30.0–37.6% and immature platelet fraction was 0.8–5.6%. There was a statistically significant difference (p-value = 0.006) between genders in respect to the immature platelet fraction with 0.8–4.7% for females and 0.7–6.1% for males. The immature reticulocyte fraction was directly correlated with the reticulocyte count. Determining the reference range is critical to the introduction of a new parameter. The reference ranges obtained herein corroborate those reported in previous publications and will contribute to the clinical and laboratory application of the indices.