New pulsatile bioreactor for in vitro formation of tissue engineered heart valves
New pulsatile bioreactor for in vitro formation of tissue engineered heart valves
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DOI：
10.1089/107632700320919
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发表时间：
2000-02-01
影响因子：
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通讯作者：
Mayer, JE
中科院分区：
文献类型：
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作者：
Hoerstrup, SP;Sodian, R;Mayer, JE
制造可植入组织工程心脏瓣膜的两个潜在障碍是机械性能不足（承受血流动力学应力的能力）和由于残留的未降解聚合物支架的存在而引起的不良宿主组织反应。为了解决这些问题，我们开发了一种体外细胞培养系统，为发育中的瓣膜结构提供生理压力和营养培养基流。预计体外物理应力将刺激组织工程心脏瓣膜构造在可能的植入之前产生足够的强度。长期的体外开发将通过一个隔离的、因而抗污染的系统来实现。更长的体外开发将有可能在体外培养过程中使聚合物支架实现更完全的生物降解。这种新型动态生物反应器允许调节脉动流和变化的压力水平。该系统结构紧凑，可轻松装入标准细胞培养箱，代表高度隔离的动态细胞培养环境，具有最高的无菌度、最佳的气体供应和稳定的温度条件，特别适合长期实验。
Two potential obstacles to the creation of implantable tissue engineered heart valves are inadequate mechanical properties (ability to withstand hemodynamic stresses) and adverse host-tissue reactions due to the presence of residual nondegraded polymer scaffold. In an attempt to address these problems, we developed an in vitro cell culture system that provides physiological pressure and flow of nutrient medium to the developing valve constructs. It is anticipated that in vitro physical stress will stimulate the tissue engineered heart valve construct to develop adequate strength prior to a possible implantation. Long-term in vitro development will be realized by an isolated and thereby contamination-resistant system. Longer in vitro development will potentially enable more complete biodegradation of the polymeric scaffold during in vitro cultivation. This new dynamic bioreactor allows for adjustable pulsatile flow and varying levels of pressure. The system is compact and easily fits into a standard cell incubator, representing a highly isolated dynamic cell culture setting with maximum sterility, optimal gas supply and stable temperature conditions especially suited for long-term experiments.