Histological Study on the Protective Effect of Endogenous Stem Cell Mobilization in Busulfan-Induced Testicular Injury in Albino Rats.
Histological Study on the Protective Effect of Endogenous Stem Cell Mobilization in Busulfan-Induced Testicular Injury in Albino Rats.
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DOI：
10.4103/jmau.jmau_35_18
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发表时间：
2018-10
影响因子：
--
通讯作者：
Mohamed HH
中科院分区：
文献类型：
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作者：
Aboul Fotouh GI;Abdel-Dayem MM;Ismail DI;Mohamed HH
睾丸损伤是化疗最危险的影响之一，因为它经常与少精子症和无精子症有关。本研究旨在评价粒细胞集落刺激因子（G-CSF）动员造血干细胞对白消安所致大鼠睾丸损伤的保护作用。将24只成年白化病大鼠分为4组：I组为对照组，II组为白消安腹腔注射（IP），间隔14天，III组为白消安腹腔注射，IV组为白消安腹腔注射后连续5天皮下注射G-CSF（70 μg/kg/day）。睾丸切片用H和E染色，并进行CD 34、增殖细胞核抗原（PCNA）和caspase-3的免疫组化染色，半薄切片用甲苯胺蓝染色。组II和组III显示睾丸和生精细胞的正常组织结构丧失，凋亡增加，证实了caspase-3的显着增加和PCNA免疫表达显着降低。而IV组显示睾丸组织学改善，凋亡减少，生精细胞增殖能力增加。caspase-3免疫表达显著降低，PCNA免疫反应显著增加。发现用G-CSF动员干细胞可改善白化病大鼠白消安化疗后的睾丸组织学。
Testicular damage is one of the most hazardous effects of chemotherapy as it is frequently associated with oligozoospermia and azoospermia. This study aimed at evaluating the protective effect of hematopoietic stem cell mobilization by granulocyte colony-stimulating factor (G-CSF) in a rat model of busulfan-induced testicular injury. Twenty-four adult albino rats were divided into four groups: group I, the control, Group II: rats received two doses of busulfan (each 15 mg/kg) intraperitoneally (IP) with 14 days interval, Group III: rats received busulfan and left untreated, and Group IV received busulfan IP then G-CSF (70 μg/kg/day) subcutaneously for 5 consecutive days. Testicular sections were stained with H and E and immunohistochemically for CD34, proliferating cell nuclear antigen (PCNA) and caspase-3, and semithin sections were stained with toluidine blue. Groups II and III showed loss of the normal histological architecture of the testis and spermatogenic cells, with increased apoptosis confirmed by significantly increased caspase-3 and significantly decreased PCNA immunoexpression. While Group IV revealed improved testicular histology, decreased apoptosis, and increased proliferative capacity of spermatogenic cells. This was confirmed by significantly decreased caspase-3 immunoexpression and increased PCNA immunoreaction. Mobilization of stem cells with G-CSF was found to improve the testicular histology following busulfan chemotherapy in albino rats.