Production of fluorous-based microarrays with uncharged carbohydrates.

Production of fluorous-based microarrays with uncharged carbohydrates.
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使用不带电荷的碳水化合物生产基于氟的微阵列。

DOI:
10.1007/978-1-61779-373-8_10
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发表时间:
2012
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
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通讯作者:
Pohl,NicolaLB
Pohl,NicolaLB
中科院分区:
--
文献类型:
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作者:
Nagappayya,SahanaK;Pohl,NicolaLB

文献摘要

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Fluorous-based carbohydrate microarrays provide an alternative to traditional covalent microarray platforms for probing protein–carbohydrate-binding interactions. The most studied plant lectin, concanavalin A (ConA), is known to bind to terminally α-linked mannose. In the studies presented, the binding of ConA with α-mannose is analyzed using a microarray formed on a fluorous-coated glass slide with the sugar containing a fluorous tag at the anomeric position.