Morphology, growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines.
Morphology, growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines.
复制标题
两种新的人神经母细胞瘤细胞系的形态、生长、染色体模式和纤溶活性。
作者：
Robert C. Seeger;Sylvia A. Rayner;Ashu Banerjee;Helen Chung;W. Laug;Harry B. Neustein;William F. Benedict
神经母细胞瘤细胞系LA-N-1和LA-N-2分别从两名患有转移性神经母细胞瘤的儿童的骨髓和原发肿瘤中的神经母细胞瘤细胞中建立。形态，生长在体外和无胸腺裸鼠，染色体图案，和纤溶活性的这些细胞系和以前建立的人神经母细胞瘤细胞系IMR-32，SK-N-MC，SK-N-SH进行了比较。大多数LA-N-1细胞为泪滴状，小细胞，有突起;它们倾向于成簇生长。LA-N-2由细长细胞和小圆形细胞组成，后者在前者上密集生长。电镜下可见LA-N-1细胞胞质内有大量致密核，而LA-N-2细胞无致密核。在体外对数生长期间，LA-N-1、LA-N-2、SK-N-MC、SK-N-SH和IMR-32细胞的倍增时间分别为32、56、23、36和26小时。所有细胞系的细胞在软琼脂中形成集落，并且在可变的潜伏期后，LA-N-1、LA-N-2、SK-N-MC和IMR-32细胞在无胸腺裸鼠中形成肿瘤。每个细胞系的标记染色体特征存在于给定细胞系90%以上的细胞中。显著的纤溶酶原依赖性纤溶活性存在于所有细胞系的细胞中。这些研究表明，LA-N-1和LA-N-2细胞来自单一但不同的异常祖细胞，并且它们具有神经母细胞瘤细胞的性质。他们还证明，来自人类神经母细胞瘤的细胞系是异质性的，就像儿童肿瘤一样。
Neuroblastoma cell lines LA-N-1 and LA-N-2 were extablished from neuroblastoma cells in the bone marrow and in the primary tumor, respectively, of two children with metastatic neuroblastoma. Morphology, growth in vitro and in athymic nude mice, chromosomal patter, and fibrinolytic activity of these cell lines and of previously extablished human neuroblastoma cell lines IMR-32, SK-N-MC, and SK-N-SH were compared. Most LA-N-1 cells were tear-drop shaped, small cells with processes; they tended to grow in clusters. LA-N-2 was comprised of elongated cells and small round cells, the latter growing in dense clumps on the former. Electron microscopy revealed numerous cytoplasmic dense cores in many LA-N-1 cells but none in LA-N-2 CELLS. During logarithmic growth in vitro, doubling times for LA-N-1, LA-N-2, SK-N-MC, SK-N-SH, and IMR-32 cells were 32,56, 23, 36, and 26 hr, respectively. Cells of all lines formed colonies in soft agar, and, after variable latency periods, LA-N-1, LA-N-2, SK-N-MC, and IMR-32 cells formed tumors in athymic nude mice. The marker chromosome(s) characteristic of each cell line was present in more than 90% of cells of given line. Significant plasminogen-dependent fibrinolytic activity was present in cells of all lines. These studies indicate that LA-N-1 and LA-N-2 cells arose from single but different aberrant progenitor cells and that they have properties of neuroblastoma cells. They also demonstrate that cell lines derived from human neuroblastomas are heterogenous as are the tumors in children.