Lineage tracing quantification reveals symmetric stem cell division in Drosophila male germline stem cells.
Lineage tracing quantification reveals symmetric stem cell division in Drosophila male germline stem cells.
复制标题
谱系追踪定量揭示了果蝇雄性生殖干细胞的对称干细胞分裂。
DOI：
10.1007/s12195-013-0295-6
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发表时间：
2013
影响因子：
2.8
通讯作者：
Yamashita,YukikoM
中科院分区：
文献类型：
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作者：
Salzmann,Viktoria;Inaba,Mayu;Cheng,Jun;Yamashita,YukikoM
在体内平衡状态下，成体干细胞对称分裂以增加干细胞数量以补偿干细胞损失，或不对称分裂以维持群体同时产生分化细胞。我们用谱系追踪的方法研究了果蝇睾丸干细胞的分裂方式，证实了该系统中存在对称性干细胞分裂。我们发现，对称分裂的速率被限制在总生殖系干细胞分裂的1-2%，但是它随着细胞粘附分子E-cadherin或肌动蛋白细胞骨架的调节因子Moesin的表达而增加，这可能调节生殖细胞向干细胞龛的迁移。我们的研究结果表明，关于不对称与对称分裂的决定是一个动态调节的过程，有助于组织的稳态，响应组织的需要。
In the homeostatic state, adult stem cells divide either symmetrically to increase the stem cell number to compensate stem cell loss, or asymmetrically to maintain the population while producing differentiated cells. We have investigated the mode of stem cell division in the testes ofDrosophila melanogasterby lineage tracing and confirm the presence of symmetric stem cell division in this system. We found that the rate of symmetric division is limited to 1–2% of total germline stem cell divisions, but it increases with expression of a cell adhesion molecule, E-cadherin, or a regulator of the actin cytoskeleton, Moesin, which may modulate adhesiveness of germ cells to the stem cell niche. Our results indicate that the decision regarding asymmetric vs. symmetric division is a dynamically regulated process that contributes to tissue homeostasis, responding to the needs of the tissue.