Label-Free Free-Solution Single-Molecule Protein Small Molecule Interaction Observed by Double-Nanohole Plasmonic Trapping
Label-Free Free-Solution Single-Molecule Protein Small Molecule Interaction Observed by Double-Nanohole Plasmonic Trapping
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DOI：
10.1021/ph5000314
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发表时间：
2014-05-01
期刊：
影响因子：
7
通讯作者：
Gordon, Reuven
中科院分区：
文献类型：
--
作者：
Al Balushi, Ahmed A.;Gordon, Reuven
蛋白质与小分子的相互作用是它们在活生物体中发挥功能的基础，并且在药物开发中被广泛研究。在这里，我们使用双纳米孔光捕获技术，观察实时无标记的自由溶液单分子动力学的三个复合物：生物素链霉亲和素，生物素单价链霉亲和素，和乙酰水杨酸环氧合酶2。在具有和不具有小分子结合的蛋白质之间观察到根本不同的行为。该检测平台具有可扩展性、廉价性和高度灵敏性，这可能会改变基于蛋白质小分子相互作用的药物发现。
The interaction of proteins with small molecules is fundamental to their 100 function in living organisms, and it is widely studied in drug development. Here we use the double nanohole optical trapping technique to observe real-time label-free free-solution single-molecule dynamics of three complexes: biotin streptavidin, biotin monovalent streptavidin, and acetylsalicylic acid cyclooxygenase 2. Radically different behavior is seen between the protein with and without the small molecule binding. This detection platform is scalable, inexpensive, and highly sensitive, which may transform drug discovery based on protein small molecule interactions.