Epidermal lesions of the GVHR: evaluation of the role of different MHC and non MHC loci and of the Ly-2+ and L3T4+ T lymphocytes.
Epidermal lesions of the GVHR: evaluation of the role of different MHC and non MHC loci and of the Ly-2+ and L3T4+ T lymphocytes.
复制标题
GVHR 的表皮病变：评估不同 MHC 和非 MHC 位点以及 Ly-2 和 L3T4 T 淋巴细胞的作用。
DOI：
10.4049/jimmunol.139.2.406
复制
发表时间：
1987
影响因子：
4.4
通讯作者：
J. Saurat
中科院分区：
文献类型：
--
作者：
P. Piguet;A. Janin;P. Vassalli;J. Saurat
辐照小鼠（750拉德）注射T-耗尽骨髓细胞（BMC）和T淋巴细胞的各种组合的T/宿主不相容。表皮进行组织学检查和两个基本的表皮病变的移植物抗宿主病（GVHD），表皮细胞坏死（ECN）和苔藓样增生反应（LR）的发生率进行了半定量评价。在急性期GVH反应（GVHR）（第15至25天），有一个明显的增加，ECN引起的反应的次要位点，整个主要组织相容性复合体（MHC）的差异，或MHC I类或II类的差异。无T淋巴细胞/表皮不相容的同种异体效应未诱导显著的ECN发生率。通过单克隆抗体处理（在体外进行，注射前或通过接受者处理），Ly-2+和L3 T4 + T淋巴细胞亚群的消耗都不能防止ECN的发生，表明两种T淋巴细胞亚群都能够引发表皮细胞损伤。LR主要是由于L3 T4+，Ly-2-辅助表型的T淋巴细胞。在Ly-2+或L3 T4+淋巴细胞诱发的慢性GVHR（35天后）中，ECN和LR不再明显，但表皮细胞的数量，尤其是表皮细胞间的复制细胞数量明显减少。
Irradiated mice (750 rad) were injected with T-depleted bone marrow cells (BMC) and T lymphocytes in various combinations of T/host incompatibility. The epidermis was examined histologically and the incidence of two basic epidermal lesions of graft-vs-host disease (GVHD), the epidermal cell necrosis (ECN) and the lichenoid hyperplastic reaction (LR), were evaluated by a semi-quantitative evaluation. During the acute phase of GVH reaction (GVHR) (days 15 to 25), there was an obvious increase in ECN in reactions elicited by minor loci, whole major histocompatability complex (MHC) differences, or a MHC class I or II difference only. Allogeneic effect without T lymphocyte/epidermis incompatibility did not induce a significant incidence of ECN. Neither depletion of the Ly-2+ nor that of the L3T4+ T lymphocyte subset by treatment with monoclonal antibody (performed in vitro, before injection or also by treatment of the recipient) did prevent the occurrence of ECN, indicating that both T lymphocyte subsets are capable of initiating the epidermal cell damage. The LR was due mainly to the T lymphocytes of the L3T4+, Ly-2- helper phenotype. During chronic GVHR (after 35 days) elicited by either Ly-2+ or L3T4+ lymphocytes, ECN and LR were no longer evident, but the number of epidermal cells and especially the number of replicating cells among the epidermal cells were markedly reduced.