Direct measurement of transmural laminar architecture in the anterolateral wall of the ovine left ventricle: new implications for wall thickening mechanics
Direct measurement of transmural laminar architecture in the anterolateral wall of the ovine left ventricle: new implications for wall thickening mechanics
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DOI：
10.1152/ajpheart.00813.2004
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发表时间：
2005-03-01
影响因子：
4.8
通讯作者：
Miller, DC
中科院分区：
文献类型：
--
作者：
Harrington, KB;Rodriguez, F;Miller, DC
绵羊左心室前外侧壁透壁层状结构的直接测量：室壁增厚力学的新含义。美国生理学杂志心脏循环生理学288：H1324-H1330，2005年。首次发表于2004年11月18日; doi：10.1152/ajpheart.00813.2004。左心室（LV）的层状或片状结构是正常收缩期和舒张期LV动力学的结构基础，但透壁片取向仍不完全表征。我们直接测量了绵羊左心室前外侧壁的跨壁面角分布。将10只Dorset杂种羊心脏用5%戊二醛缓冲液原位灌注固定，并保存在10%福尔马林中。切除心肌组织的连续块，切割边缘平行于局部周向、纵向和径向轴，并从心外膜到心内膜平行于心外膜切面切成1 mm厚的切片。平均纤维方向确定在每个部分从五个测量的纤维角。然后横向于纤维方向切割每个部分，并测量五个片材角（β）并取平均值。平均纤维角从心外膜的-41度（SD 11）到心内膜的+42度（SD 16）几乎呈线性增长。在大约+45度（β（+））和-45度（β（-））处识别出两个片材家族。在外侧区（n = 5），靠近心外膜，片属于β（+）家族;在中壁，属于β（-）家族;靠近内膜，属于β（+）家族。这种模式在基底前区（n = 4）被逆转。前乳头肌上的薄片均匀为β（-）（n = 2）。这些直接测量的纸张角度揭示，第一次，交替的透壁家庭占主导地位的纸张角度。这可能对理解正常和衰竭心脏的壁力学具有重要意义。
Direct measurement of transmural laminar architecture in the anterolateral wall of the ovine left ventricle: new implications for wall thickening mechanics. Am J Physiol Heart Circ Physiol 288: H1324-H1330, 2005. First published November 18, 2004; doi:10.1152/ajpheart.00813.2004.-Laminar, or sheet, architecture of the left ventricle (LV) is a structural basis for normal systolic and diastolic LV dynamics, but transmural sheet orientations remain incompletely characterized. We directly measured the transmural distribution of sheet angles in the ovine anterolateral LV wall. Ten Dorsett-hybrid sheep hearts were perfusion fixed in situ with 5% buffered glutaraldehyde at end diastole and stored in 10% formalin. Transmural blocks of myocardial tissue were excised, with the edges cut parallel to local circumferential, longitudinal, and radial axes, and sliced into 1-mm-thick sections parallel to the epicardial tangent plane from epicardium to endocardium. Mean fiber directions were determined in each section from five measurements of fiber angles. Each section was then cut transverse to the fiber direction, and five sheet angles (beta) were measured and averaged. Mean fiber angles progressed nearly linearly from -41degrees (SD 11) at the epicardium to +42degrees ( SD 16) at the endocardium. Two families of sheets were identified at approximately +45degrees (beta(+)) and -45degrees (beta(-)). In the lateral region (n = 5), near the epicardium, sheets belonged to the beta(+) family; in the midwall, to the beta(-) family; and near the endocardium, to the beta(+) family. This pattern was reversed in the basal anterior region (n = 4). Sheets were uniformly beta(-) over the anterior papillary muscle (n = 2). These direct measurements of sheet angles reveal, for the first time, alternating transmural families of predominant sheet angles. This may have important implications in understanding wall mechanics in the normal and the failing heart.