Auxin binding protein 1 (ABP1) is not required for either auxin signaling or Arabidopsis development
Auxin binding protein 1 (ABP1) is not required for either auxin signaling or Arabidopsis development
复制标题
DOI：
10.1073/pnas.1500365112
复制
发表时间：
2015-02-17
影响因子：
11.1
通讯作者：
Zhao, Yunde
中科院分区：
文献类型：
--
作者：
Gao, Yangbin;Zhang, Yi;Zhao, Yunde
生长素结合蛋白1(ABP1)已被研究了几十年。已有研究表明，ABP1是生长素受体，在许多发育过程中起着重要的作用。在这里，我们提出了我们意想不到的发现，ABP1既不是生长素信号所必需的，也不是正常生长条件下植物发育所必需的。我们使用我们的基于核酶的CRISPR技术来产生一个拟南芥abp1突变体，该突变体在ABP1的第一个外显子上包含一个5-bp的缺失，这导致了码移和早期终止密码子的引入。我们还鉴定了一个T-DNA插入abp1等位基因，该等位基因位于第一外显子ATG起始密码子下游27bp处。我们证明这两个新的abp1突变体是零等位基因。令人惊讶的是，我们新的abp1突变植株没有表现出任何明显的发育缺陷。事实上，在分析的每个发育阶段，突变植物与野生型植物是难以区分的。此外，abp1植株对外源生长素没有抗性。在分子水平上，我们发现已知的生长素调节基因的诱导在野生型和abp1植物中对生长素处理的响应是相似的。我们得出的结论是，ABP1不是生长素信号或拟南芥发育的关键成分。
Auxin binding protein 1 (ABP1) has been studied for decades. It has been suggested that ABP1 functions as an auxin receptor and has an essential role in many developmental processes. Here we present our unexpected findings that ABP1 is neither required for auxin signaling nor necessary for plant development under normal growth conditions. We used our ribozyme-based CRISPR technology to generate an Arabidopsis abp1 mutant that contains a 5-bp deletion in the first exon of ABP1, which resulted in a frameshift and introduction of early stop codons. We also identified a T-DNA insertion abp1 allele that harbors a T-DNA insertion located 27 bp downstream of the ATG start codon in the first exon. We show that the two new abp1 mutants are null alleles. Surprisingly, our new abp1 mutant plants do not display any obvious developmental defects. In fact, the mutant plants are indistinguishable from wild-type plants at every developmental stage analyzed. Furthermore, the abp1 plants are not resistant to exogenous auxin. At the molecular level, we find that the induction of known auxin-regulated genes is similar in both wild-type and abp1 plants in response to auxin treatments. We conclude that ABP1 is not a key component in auxin signaling or Arabidopsis development.