In vitro study on metabolite profiles of bioactive xanthones isolated from Halenia elliptica D. Don by high performance liquid chromatography coupled to ion trap time-of-flight mass spectrometry
In vitro study on metabolite profiles of bioactive xanthones isolated from Halenia elliptica D. Don by high performance liquid chromatography coupled to ion trap time-of-flight mass spectrometry
复制标题
通过高效液相色谱结合离子阱飞行时间质谱法对从 Halenia elliptica D. Don 中分离的生物活性呫吨酮的代谢谱进行体外研究
DOI：
10.1016/j.jpba.2012.01.014
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发表时间：
2012-03-25
影响因子：
3.4
通讯作者：
Zhang, Yu-Kui
中科院分区：
文献类型：
--
作者：
Feng, Ru;Zhang, Yi-Ying;Zhang, Yu-Kui
对1-羟基-2,3,5-三甲氧基口山酮（HM-1）、1-羟基-2,3,4,7-四甲基口山酮（HM-2）、1-羟基-2,3,4,5-四甲基口山酮（HM-3）、1,7-二羟基-2,3,4,5-四甲基口山酮（HM-4）和1,5-二羟基-2,3-二甲氧基口山酮（HM-5）在体外大鼠肝微粒体中的作用进行了研究。采用高效液相色谱-离子阱飞行时间质谱法（LC-ESI-IT-TOF）鉴定了上述5种口山酮的代谢物，并用H-1 NMR对主要代谢物进行了鉴定。鉴定了13种代谢物的结构，其中7种以前未报道过。此外，还可以通过不同阶段或相对丰度的断裂行为来区分山酮异构体。结果表明，HM-1、HM-2、HM-3、HM-4和HM-5的体外代谢转化主要发生在母体药物结构上的2-、4-、5-、7-碳位置。代谢物可能是新的血管活性物质。(C) 2012 Elsevier B.V.版权所有
The metabolisms of five xanthones isolated from a Tibetan medicinal herb Halenia elliptica D. Don, including 1-hydroxy-2,3,5-trimethoxy-xanthone (HM-1), 1-hydroxy-2,3,4,7-tetramethoxy-xanthone (HM-2), 1-hydroxy-2,3,4,5-tetramethoxy-xanthone (HM-3), 1,7-dihydroxy-2,3,4,5-tetramethoxy-xanthone (HM-4) and 1,5-dihydroxy-2,3-dimethoxy-xanthone (HM-5), were studied in rat liver microsomes in vitro. High performance liquid chromatography coupled to ion trap time-of-flight mass spectrometry (LC-ESI-IT-TOF) was applied for identification of metabolites of five xanthones mentioned above and H-1 NMR was used to elucidate the major metabolites. The structures of thirteen metabolites were identified and seven of them had not been reported before. Moreover, xanthone isomers herein could be distinguished by difference of fragmentation behaviors with increase of stages or relative abundances. The results indicated that in vitro metabolic transformation of HM-1, HM-2, HM-3, HM-4 and HM-5 occurred mainly at 2-, 4-, 5-, 7-carbonic positions on their structures of parent drugs. The metabolites could be new vasoactive substances. This work will provide a basis for study on the structure-activity relationships of these xanthones and their derivatives from Tibetan herbal in the next work. (C) 2012 Elsevier B.V. All rights reserved.