Interleukin-1beta increases elasticity of human bioartificial tendons.
Interleukin-1beta increases elasticity of human bioartificial tendons.
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DOI：
10.1089/ten.2006.12.2913
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发表时间：
2006-10
影响因子：
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通讯作者：
J. Qi;Liqun Chi;M. Maloney;Xi Yang;D. Bynum;A. Banes
中科院分区：
文献类型：
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作者：
J. Qi;Liqun Chi;M. Maloney;Xi Yang;D. Bynum;A. Banes
刚度是结缔组织的一项重要力学性能，特别是对体内受循环应变的组织，如肌腱。因此，调节天然组织或工程组织的材料特性是组织修复的重要考虑因素。白细胞介素1- β (il -1 β)是结缔组织中最常与基质金属蛋白酶诱导和基质破坏相关的细胞因子。然而，il -1 β也可能参与建设性重塑并赋予细胞存活价值。在这项研究中，我们研究了il -1 β对在新型三维（3D）培养系统中制备的人类腱细胞填充生物人工肌腱（BATs）性能的影响。il -1 β处理降低了bat的极限拉伸强度和弹性模量，增加了最大应变。il -1 β在低剂量（1,10 pM）下上调弹性蛋白表达，在高剂量（100 pM）下下调I型胶原表达。参与基质重塑的基质金属蛋白酶也被il -1 β上调。增加的弹性防止了蝙蝠因施加的应变而破裂。本研究的结果表明，il -1 β可能作为一种防御/生存因子，以应对施加的机械负荷。细胞内应变和外基质应变之间的平衡对维持肌腱的完整性至关重要。
Stiffness is an important mechanical property of connective tissues, especially for tissues subjected to cyclic strain in vivo, such as tendons. Therefore, modulation of material properties of native or engineered tissues is an important consideration for tissue repair. Interleukin 1-beta (IL-1beta) is a cytokine most often associated in connective tissues with induction of matrix metalloproteinases and matrix destruction. However, IL-1beta may also be involved in constructive remodeling and confer a cell survival value to tenocytes. In this study, we investigated the effects of IL-1beta on the properties of human tenocyte-populated bioartificial tendons (BATs) fabricated in a novel three-dimensional (3D) culture system. IL-1beta treatment reduced the ultimate tensile strength and elastic modulus of BATs and increased the maximum strain. IL-1beta at low doses (1, 10 pM) upregulated elastin expression and at a high dose (100 pM) downregulated type I collagen expression. Matrix metalloproteinases, which are involved in matrix remodeling, were also upregulated by IL-1beta. The increased elasticity prevented BATs from rupture caused by applied strain. The results in this study suggest that IL-1beta may act as a defense/survival factor in response to applied mechanical loading. The balance between cell intrinsic strain and external matrix strain is important for maintaining the integrity of tendons.