HEMOPHILUS-INFLUENZAE BACTEREMIA AND MENINGITIS RESULTING FROM SURVIVAL OF A SINGLE ORGANISM
HEMOPHILUS-INFLUENZAE BACTEREMIA AND MENINGITIS RESULTING FROM SURVIVAL OF A SINGLE ORGANISM
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DOI：
10.1073/pnas.75.3.1534
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发表时间：
1978-01-01
影响因子：
11.1
通讯作者：
MURPHY, PA
中科院分区：
文献类型：
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作者：
MOXON, ER;MURPHY, PA
用链霉素敏感株和链霉素耐药株的混合物鼻内感染幼鼠。采集鼻咽冲洗液、血液和脑脊液，进行B型流感病毒培养。如果在建立感染过程中感染微生物相互合作，鼻咽、血液和CSF培养物应含有变异体的混合物。如果每种微生物都是独立作用的，那么用少量的感染接种物，鼻咽部、血液或CSF中的所有微生物都应该是从一种细菌传下来的。然后培养物应仅包含其中一种变体。当体内接种物<100个微生物时，19只大鼠中有8只（42%）获得了单一变异的鼻咽培养物。随着接种物的增加，观察到单一变异培养物的频率降低。当接种物≥ 100 μ l时，105种微生物，鼻咽培养物总是混合物。当大鼠鼻内接种108种微生物时，在67次菌血症发作中的46次（68.7%）中获得了单一变异血培养。从19只（68.4%）脑膜炎大鼠的脑脊液中分离出单一变体，其血液中含有链霉素敏感和链霉素耐药变体。当血液中含有单一变异体时，在40次中的39次（97.5%）从CSF中培养出相同的变异体。入侵H.与鼻内接种微生物的协同相互作用相反，幼年大鼠的流感B型感染是由独立作用引起的。脑膜明显受到血行途径的侵袭。
Infant rats were infected intranasally with mixtures of streptomycin-sensitive and streptomycin-resistant strains of H. influenzae type b and cultures of nasopharyngeal washings, blood and CSF fluid were obtained. If the infecting organisms cooperated with each other during the establishment of infection, nasopharyngeal, blood and CSF cultures should have contained mixtures of the varients. If each organism acted independently, then with small infecting inocula all the organisms in nasopharynx, blood or CSF should be descended from a single bacterium. Cultures should then contain only 1 of the variants. Single variant nasopharyngeal cultures were obtained from 8 out of 19 (42%) rats when the intransal inoculum was < 100 organisms. As the inoculum was increased, single variant cultures were less frequently observed. When the inoculum was .gtoreq. 105 organisms, nasopharyngeal cultures were always mixtures. Single variant blood cultures were obtained in 46 of 67 (68.7%) episodes of bacteremia when rats were inoculated intranasally with 108 organisms. Single variants were isolated from the CSF fluid of 13 of 19 (68.4%) rats with meningitis whose blood contained streptomycin-sensitive and streptomycin-resistant variants. When the blood contained a single variant, this same variant was cultured from the CSF on 39 of 40 (97.5%) occasions. Invasive H. influenzae b infections of infant rats resulted from independent action, as opposed to cooperative interaction of intranasally inoculated organisms. The meninges were apparently invaded by the hematogenous route.