Structural aspects of fish skin collagen which forms ordered arrays via liquid crystalline states
Structural aspects of fish skin collagen which forms ordered arrays via liquid crystalline states
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DOI：
10.1016/s0142-9612(99)00244-6
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发表时间：
2000-05-01
期刊：
影响因子：
14
通讯作者：
Herbage, D
中科院分区：
文献类型：
--
作者：
Giraud-Guille, MM;Besseau, L;Herbage, D
从比目鱼中提取的酸溶的I型胶原蛋白在凝聚相中形成有序排列的能力已经与小牛皮胶原蛋白的数据进行了比较。通过用超声波对分子群进行初步处理，优化了体外液晶组装。这种处理要求用X射线衍射法和差示扫描量热法控制鱼胶原蛋白三螺旋结构的稳定性，用粘度测量和凝胶电泳法评价超声处理的效果。在偏光显微镜下，浓度至少为40mgml(-1)的胶原蛋白溶液在偏光显微镜下显示出典型的前胆固醇相的双折射图案，表明胶原蛋白液体相中的长程有序。将液晶胶原稳定成凝胶基质后获得的超微结构数据表明，中和的酸溶鱼胶原形成了典型的I型胶原的横纹纤维，在胆固醇前区域遵循正弦波状波动。这些有序的几何结构，近似于活体情况，赋予了材料有趣的机械性能。(C)2000爱思唯尔科学有限公司。保留所有权利。
The ability of acid-soluble type I collagen extracts from Soleidae flat fish to form ordered arrays in condensed phases has been compared with data for calf skin collagen. Liquid crystalline assemblies in vitro are optimized by preliminary treatment of the molecular population with ultrasounds. This treatment requires the stability of the fish collagen triple helicity to be controlled by X-ray diffraction and differential scanning calorimetry and the effect of sonication to be evaluated by viscosity measurements and gel electrophoresis. The collagen solution in concentrations of at least 40 mg ml(-1) showed in polarized light microscopy birefringent patterns typical of precholesteric phases indicating long-range order within the fluid collagen phase. Ultrastructural data, obtained after stabilization of the liquid crystalline collagen into a gelated matrix, showed that neutralized acid-soluble fish collagen forms cross-striated fibrils, typical of type I collagen, following sine wave-like undulations in precholesteric domains. These ordered geometries, approximating in vivo situations, give interesting mechanical properties to the material. (C) 2000 Elsevier Science Ltd. All rights reserved.