Bioluminescence Sensing of γ-Glutamyltranspeptidase Activity In Vitro and In Vivo
Bioluminescence Sensing of γ-Glutamyltranspeptidase Activity In Vitro and In Vivo
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DOI：
10.1021/acs.analchem.7b00567
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发表时间：
2017-07-04
影响因子：
7.4
通讯作者：
Liang, Gaolin
中科院分区：
文献类型：
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作者：
Hai, Zijuan;Wu, Jingjing;Liang, Gaolin
γ-谷氨酰转肽酶（GGT）是一种重要的肿瘤生物标志物，但利用生物发光（BL）探针对其活性进行真实的实时监测尚未见报道。在此，我们合理地设计了两个GGT可切割的BL探针AmLH 2（1）和Glu-p-aminobenzyloxycarbonyl-AmLH 2（2），并成功地将其应用于在体外和体内均具有高灵敏度和优异选择性的GGT活性传感。结果表明，尽管2的背景BL信号比1低，但GGT对1的催化效率比2高，并且1在活细胞和肿瘤中对GGT活性的传感上级2。我们设想，在不久的将来，outpobe 1可以广泛应用于诊断重要的GGT相关疾病的动物模型。
gamma-Glutamyltranspeptidase (GGT) is an important tumor biomarker but using a bioluminescence (BL) probe to real time monitor its activity as not been repotted. Herein, we rationally designed two GGT-cleavable BL probes AmLH2 (1) and Glu-p-aminobenzyloxycarbonyl-AmLH2 (2), and successfully applied them for sensing GGT activity with high sensitivity and excellent selectivity both in vitro and in vivo. The results indicated that, although, 2 had lower background BL signal than 1, GGT had higher catalytic efficiency for 1 than 2, and 1 was superior to 2 for sensing GGT activity in living cells and tumors. We envision that out ptobe 1 could be widely applied for the diagnosis of important GGT-related diseases in animal models in the near future.