UNUSUAL CYTOSKELETAL AND CHROMATIN CONFIGURATIONS IN MOUSE OOCYTES THAT ARE ATYPICAL IN MEIOTIC PROGRESSION
UNUSUAL CYTOSKELETAL AND CHROMATIN CONFIGURATIONS IN MOUSE OOCYTES THAT ARE ATYPICAL IN MEIOTIC PROGRESSION
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DOI：
10.1002/dvg.1020160105
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发表时间：
1995-01-01
期刊：
影响因子：
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通讯作者：
EPPIG, JJ
中科院分区：
文献类型：
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作者：
ALBERTINI, DF;EPPIG, JJ
LT/Sv 和 I/LnJ 小鼠品系的卵母细胞减数分裂成熟进展异常。 LT/Sv occytes 显示出高频率的中期 I 停滞和孤雌激活。 I/LnJ 卵母细胞表现出减数分裂成熟动力学迟缓和中期 I 停滞频率高。一些 I/LnJ 卵母细胞无法恢复减数分裂。染色质、微管和中心体结构的变化与减数分裂进展的特定阶段相关。在这项研究中，在 LT/Sv、I/LnJ 和 C57BL/6J（对照）卵母细胞中检查了这些亚细胞成分的配置，这些卵母细胞要么是从大窦卵泡中新鲜分离的，要么是在培养 15 小时以允许自发减数分裂成熟后进行的。与对照卵母细胞相比，LT/Sv 和 I/LnJ 卵母细胞的染色质、微管和中心体的组织存在差异。例如，LT/Sv 卵母细胞通常含有单个大中心体，而不是像正常生发囊泡阶段卵母细胞那样表现出多个细胞质和核中心体。相比之下，I/LnJ 卵母细胞显示出许多小的中心体。正常生发囊泡期卵母细胞的微管组织为阵列或紫苑，但 LT/Sv 卵母细胞中的微管较短，而 I/LnJ 卵母细胞中不存在微管。培养 15 小时后，正常中期 II 卵母细胞的中心体材料在两个纺锤体极上组织起来。相比之下，中期 I 停滞的 LT/Sv 卵母细胞表现出细长的纺锤体，中心体物质在纺锤体的一极显得更有组织。对照和 LT/Sv 卵母细胞均显示细胞质中心体。中期 I 停滞的 I/LnJ 卵母细胞很少有细胞质中心体，但在纺锤体外围表现出中心体病灶。因此，在减数分裂成熟过程中非典型的卵母细胞表现出微管和中心体的异常构型，这被认为参与了减数分裂成熟的调节。 (C) 1995 Wiley-Liss, Inc.
Meiotic maturation progresses atypically in oocytes of strain LT/Sv and I/LnJ mice. LT/Sv occytes show a high frequency of metaphase I-arrest and parthenogenetic activation. I/LnJ oocytes display retarded kinetics of meiotic maturation and a high frequency of metaphase I-arrest. Some I/LnJ oocytes fail to resume meiosis. Changes in the configuration of chromatin, microtubules, and centrosomes are associated with specific stages of meiotic progression. In this study, the configuration of these subcellular components was examined in LT/Sv, I/LnJ, and C57BL/6J (control) oocytes either freshly isolated from large antral follicles or after culture for 15 hr to allow progression of spontaneous meiotic maturation. Differences were found in the organization of chromatin, microtubules, and centrosomes in LT/Sv and I/LnJ oocytes compared to control oocytes. For example, rather than exhibiting multiple cytoplasmic and nuclear centrosomes as in the normal germinal vesicle-stage oocytes, LT/Sv oocytes typically contain a single large cencentrosome. In contrast, I/LnJ oocytes displayed many small centrosomes. The microtubules of normal germinal vesicle-stage oocytes were organized as arrays or asters, but microtubules were shorter in LT/Sv oocytes and absent from I/LnJ oocytes. After a 15-hr culture, centrosomal material of normal metaphase II oocytes was organized at both spindle poles. In contrast, metaphase I-arrested LT/Sv oocytes exhibited an elongated spindle with centrosomal material appearing more organized at one pole of the spindle. Both control and LT/Sv oocytes displayed cytoplasmic centrosomes. Metaphase I-arrested I/LnJ oocytes rarely had cytoplasmic centrosomes but exhibited centrosomal foci at the spindle periphery. Thus, oocytes that are atypical in the progression of meiotic maturation displayed aberrant configurations of microtubules and centrosomes, which are thought to participate in the regulation of meiotic maturation. (C) 1995 Wiley-Liss, Inc.