Cell renewal and gene expression in the trachea of hamster at different ages.
Cell renewal and gene expression in the trachea of hamster at different ages.
复制标题
不同年龄仓鼠气管细胞更新和基因表达。
DOI：
10.1002/ar.1092330209
复制
发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Joiakim,AP
中科院分区：
文献类型：
--
作者：
Chopra,DP;Cooney,RA;Joiakim,AP
The tracheal mucosa of the Syrian golden hamster has been extensively employed as a model system for respiratory tract cell renewal, injury, and carcinogenesis. However, baseline cell kinetic data are not available for normal juvenile and adolescent animals in which the mucosa and cartilage are rapidly enlarging. The objective of this research was to elucidate alterations in cell kinetics, epithelial morphology, and gene expression in the trachea of hamsters at different ages. Cell kinetics were examined by3H‐thymidine labeling indices, morphology by light and electron microscopic examination, and gene expression by slot blot analysis. Results showed that mucosal epithelium of the young and adolescent hamster undergoes cyclic necrosis and cell shedding, exposing portions of the elastic basal lamina. Epithelial shedding was associated with hyperplasia and squamous metaplasia. Additionally, the labeling indices of mucosal epithelial cells and chondroblasts also exhibited variable patterns which were associated with a cyclic pattern of expression of c‐fos and c‐erB2 proto‐oncogenes and epidermal growth factor receptor. © 1992 Wiley‐Liss, Inc.
