THE ANTI-LIPOLYTIC EFFECT OF INSULIN IN HUMAN ADIPOSE-TISSUE IN OBESITY, DIABETES-MELLITUS, HYPERINSULINEMIA, AND STARVATION
THE ANTI-LIPOLYTIC EFFECT OF INSULIN IN HUMAN ADIPOSE-TISSUE IN OBESITY, DIABETES-MELLITUS, HYPERINSULINEMIA, AND STARVATION
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DOI：
10.1016/0026-0495(81)90020-2
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发表时间：
1981-01-01
影响因子：
9.8
通讯作者：
OSTMAN, J
中科院分区：
文献类型：
--
作者：
ARNER, P;BOLINDER, J;OSTMAN, J
在与胰岛素对葡萄糖代谢作用的抵抗相关的条件下，研究了胰岛素的体外抗脂解作用。人皮下脂肪组织取自14名肥胖受试者饥饿前和饥饿7天期间，12名未经治疗的非胰岛素依赖型糖尿病（NIDDM），6名未经治疗的胰岛素依赖型糖尿病（IDDM）和10名非肥胖对照受试者。将组织与浓度范围为1- 10，000 μ U/ml的胰岛素一起或不与胰岛素一起孵育。在基础诱导条件下测定对胰岛素的反应性（最大效应）和敏感性，因为胰岛素对去甲肾上腺素[去甲肾上腺素]刺激的脂解具有双峰效应。在正常情况下，胰岛素敏感性和胰岛素反应性均与基础脂解率呈正相关。在肥胖、胰岛素依赖型糖尿病和非胰岛素依赖型糖尿病中，胰岛素敏感性和胰岛素反应性没有变化。当肥胖受试者被分为1个高胰岛素血症组（6人）和1组空腹血清胰岛素水平正常（7人）时，在2组中观察到胰岛素的相似的抗脂肪分解作用。在饥饿期间，胰岛素敏感性增加了20倍（P < 0.01），但股脂肪的胰岛素反应性没有变化，腹部脂肪的胰岛素反应性仅下降（P < 0.01）。人类脂肪细胞中的抗脂解作用显然与肥胖、饥饿、糖尿病和高胰岛素血症中的胰岛素抵抗无关。
The antilipolytic effect of insulin in vitro was investigated in conditions associated with resistance to the effect of insulin on glucose metabolism. Human subcutaneous adipose tissue was obtained from 14 obese subjects before and during starvation for 7 days, 12 untreated non-insulin dependent diabetics (NIDDM), 6 untreated insulin dependent diabetics (IDDM) and 10 nonobese control subjects. The tissue was incubated with and without insulin in concentration ranging from 1-10,000 .mu.U/ml. Responsiveness (maximum effect) and sensitivity to insulin were determined under basal induction conditions, since insulin had a bimodal effect on noradrenaline [norepinephrine] stimulated lipolysis. Under normal conditions both insulin sensitivity and insulin responsiveness were positively correlated with the basal rate of lipolysis. In obesity, IDDM and NIDDM there were no changes in insulin sensitivity or in insulin responsiveness. When the obese subjects were divided into 1 hyperinsulinemic group (6 individuals) and 1 group with normal fasting serum insulin levels (7 individuals) a similar antilipolytic effect of insulin was observed in the 2 groups. During starvation there was a 20-fold increase in insulin sensitivity (p < 0.01) but no change in insulin responsiveness in femoral fat and only a decrease in responsiveness (P < 0.01) in abdominal fat. Antilipolysis in human fat cells evidently is not involved in the insulin resistance seen in obesity, starvation, diabetes and hyperinsulinemia.