REGULATION OF PHOSPHATIDATE PHOSPHATASE-ACTIVITY BY INOSITOL IN SACCHAROMYCES-CEREVISIAE
REGULATION OF PHOSPHATIDATE PHOSPHATASE-ACTIVITY BY INOSITOL IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1128/jb.170.8.3561-3566.1988
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发表时间：
1988-08-01
影响因子：
3.2
通讯作者：
CARMAN, GM
中科院分区：
文献类型：
--
作者：
MORLOCK, KR;LIN, YP;CARMAN, GM
在补充磷脂前体的酿酒酵母细胞中检查磷脂酸磷酸酶（EC 3.1.34）活性的调节。在野生型细胞的生长培养基中添加肌醇导致磷脂酸磷酸酶活性增加两倍。磷脂酸磷酸酶活性的增加不是由于可溶性效应分子，肌醇对酶活性没有直接影响。与线粒体，微粒体，和细胞质组分的磷脂酸磷酸酶活性的调节肌醇以相同的方式。与未补充的细胞相比，补充肌醇的细胞具有升高的磷脂水平和降低的三酰甘油水平。丝氨酸，乙醇胺，胆碱没有显着影响细胞生长的磷脂酸磷酸酶活性的肌醇的存在下。肌醇生物合成调控突变体中酶活性不受调控，表明肌醇调控与肌醇生物合成调控耦合。磷脂酸磷酸酶活性在磷脂生物合成缺陷的结构基因突变体中多效性表达。结果表明，磷脂酸磷酸酶在遗传水平上受肌醇的调控。
Regulation of phosphatidate phosphatase (EC 3.1.34) activity was examined in Saccharomyces cerevisiae cells supplemented with phospholipid precursors. Addition of inositol to the growth medium of wild-type cells resulted in a twofold increase in phosphatidate phosphatase activity. The increase in phosphatidate phosphatase activity was not due to soluble effector molecules, and inositol did not have a direct effect on enzyme activity. The phosphatidate phosphatase activity associated with the mitochondrial, microsomal, and cytosolic fractions of the cell was regulated by inositol in the same manner. Cells supplemented with inositol had elevated phospholipid levels and reduced triacylglycerol levels compared with unsupplemented cells. Serine, ethanolamine, and choline did not significantly affect the phosphatidate phosphatase activity of cells growth in the absence of presence of inositol. Enzyme activity was not regulated in inositol biosynthesis regulatory mutants, suggesting that regulation by inositol is coupled to regulation by inositol biosynthesis. Phosphatidate phosphatase activity was pleiotropically expressed in structural gene mutants defective in phospholipid biosynthesis. The results suggested that phosphatidate phosphatase was regulated by inositol at a genetic level.