Ageratum conyzoides essential oil as aflatoxin suppressor of Aspergillus flavus
Ageratum conyzoides essential oil as aflatoxin suppressor of Aspergillus flavus
复制标题
DOI：
10.1016/j.ijfoodmicro.2009.10.017
复制
发表时间：
2010-01-31
影响因子：
5.4
通讯作者：
Felicio, Joana D.
中科院分区：
文献类型：
--
作者：
Nogueira, Juliana H. C.;Goncalez, Edlayne;Felicio, Joana D.
黄曲霉毒素B-1（AFB（1））是由曲霉属产生的一种高毒性和致癌代谢产物。研究了胜红蓟挥发油对黄曲霉菌丝生长和黄曲霉毒素B-1产生的抑制作用。将培养物在酵母提取物-蔗糖（YES）肉汤中于25 ℃下在以下不同浓度的精油（0.0至30 μ g/mL）下孵育数天。挥发油对真菌的生长有不同程度的抑制作用，当浓度大于0.10 μ g/mL时，可完全抑制黄曲霉毒素的产生。GC/MS分析表明，挥发油的主要成分为前茂Ⅱ（46.35%）、前茂Ⅰ（42.78%）、香豆素（5.01%）和反式-香芹烯（3.02%）。通过透射电镜比较对照和不同浓度精油培养的真菌细胞，发现超微结构的变化与精油的浓度有关。孢子虫。这种超微结构变化在内膜系统中更为明显，主要影响线粒体。在两个周围原纤维中也观察到降解。抑制黄曲霉毒素产生的能力作为一种新的生物活性表明，它可能被认为是一个有用的太]更好地了解复杂的黄曲霉毒素生物合成途径。(C)2009 Elsevier B. V.保留所有权利。
Aflatoxin B-1 (AFB(1)) is a highly toxic and carcinogenic metabolite produced by Aspergillus species on food and agricultural commodities. Inhibitory effects of essential oil of Ageratum conyzoides, on the mycelial growth and aflatoxin B-1 production by Aspergillus flavus were studied. Cultures were incubated in yeast extract-sucrose (YES) broth for days at 25 degrees C at the following different concentrations of the essential oil (from 0.0 to 30 mu g/mL). The essential oil inhibited fungal growth to different extents depending on the concentration, and completely inhibited aflatoxin production at concentrations above 0.10 mu g/mL The analysis of the oil by GC/MS showed that its main components are precocene II (46.35%), precocene I (42.78%), cumarine (5.01%) and Trans-caryophyllene (3.02%). Comparison by transmission electron microscopy of the fungal cells, control and those incubated with different concentrations of essential oil, showed ultra-structural changes which were concentration dependent of the essential oil of A. conyzoides. Such ultra-structural changes were more evident in the endomembrane system, affecting mainly the mitochondria. Degradation was also observed in both surrounding fibrils. The ability to inhibit aflatoxin production as a new biological activity of A.conyzoides L indicates that it may be considered as a useful too] for a better understanding of the complex pathway of aflatoxin biosynthesis. (C) 2009 Elsevier B.V. All rights reserved.