Rhodotorulic acid production by Rhodotorula mucilaginosa
Rhodotorulic acid production by Rhodotorula mucilaginosa
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DOI：
10.1017/s0953756203008220
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发表时间：
2003-08-01
影响因子：
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通讯作者：
Wiebe, MG
中科院分区：
文献类型：
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作者：
Andersen, D;Renshaw, JC;Wiebe, MG
粘液红酵母在铁限制条件下生长时产生铁载体红托酸（RA）。根据碳源的不同，在铁限制条件下，黏胶菇的生长速率在0.10 ~ 0.19 h（-1）之间，在铁充足条件下，生长速率为0.23 h（-1）。在用缺铁预培养物接种的生物反应器中，批培养的初始特定生长率取决于铁浓度。连续培养的临界稀释率（D-crit，稳态培养不能持续）也与铁浓度有关，低于批量培养的mu（max）。蔗糖是RA生产的最佳碳源[287 +/- 11 mumol （g生物量）（-1）]，通过在培养基中添加前体乙酸[460 +/- 13 mumol （g生物量）（-1）]、鸟氨酸[376 +/- 6 mumol （g生物量）（-1）]或两者[539 +/- 15 mumol （g生物量）（-1）]，可以进一步提高产量。柠檬酸能有效抑制RA的产生。RA的产生与生长速率有关，pH为6.5时产量最佳。
Rhodotorula mucilaginosa produces the siderophore rhodotorulic acid (RA) when grown in iron-limited conditions. R. mucilaginosa grew at rates between 0.10 and 0.19 h(-1) in iron-restricted conditions, depending on the carbon source, and at 0.23 h(-1) in iron-sufficient conditions. In bioreactors inoculated with iron-starved pre-cultures, initial specific growth rates in batch culture were dependent on the iron concentration. The critical dilution rate (D-crit, at which steady state cultures cannot be sustained) in continuous cultures was also dependent oil the iron concentration and was lower than mu(max) in batch culture. Sucrose was the best carbon source for RA production [287 +/- 11 mumol (g biomass)(-1)] and production could be further increased by supplementing the medium with the precursors acetate [460 +/- 13 mumol (g biomass)(-1)], ornithine [376 +/- 6 mumol (g biomass)(-1)], or both [539 +/- 15 mumol (g biomass)(-1)]. Citric acid was an effective suppresser of RA production. RA was produced in a growth rate dependent manner and was optimally produced at pH 6.5.