Anti-K562 cell monoclonal antibodies recognize hematopoietic progenitors.
Anti-K562 cell monoclonal antibodies recognize hematopoietic progenitors.
复制标题
抗 K562 细胞单克隆抗体可识别造血祖细胞。
DOI：
10.1073/pnas.78.11.7073
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发表时间：
1981
影响因子：
11.1
通讯作者：
S. P. Hwang
中科院分区：
文献类型：
--
作者：
N. Young;S. P. Hwang
K562白血病细胞具有与造血干细胞相似的自我更新和多能性的特性。利用杂交瘤技术制备了抗K562细胞的单克隆抗体。通过放射免疫测定，一些抗K562细胞抗体也结合红细胞抗原或外周血单核细胞;其他对K562细胞更具特异性。通过使用这些细胞在体外形成集落的能力来测定抗体与造血祖细胞的结合。人骨髓细胞暴露于抗K562抗体和补体后，髓系或红系集落形成受到抑制。通过放射免疫测定、免疫荧光和补体细胞毒性，一些抑制性抗体几乎不与成熟血细胞结合，表明它们识别未分化细胞特异性抗原。使用荧光激活细胞分选仪，一种抑制性抗体显示仅染色3%的骨髓细胞。抑制性抗K562抗体也与骨髓性白血病细胞和病毒转化的淋巴细胞结合。因此，这些抗体似乎识别正常造血祖细胞、白血病细胞和转化淋巴细胞共有的抗原。
The K562 leukemia cell has properties of self-renewal and pluripotency similar to those of the hematopoietic stem cell. Monoclonal antibodies to K562 cells have been produced by using hybridoma technology. By radioimmunoassay, some anti-K562 cell antibodies also bind to erythrocyte antigens or peripheral blood mononuclear cells; others are more specific for K562 cells. Antibody binding to hematopoietic progenitors was assayed by using the ability of these cells to form colonies in vitro. After exposure of human bone marrow cells to anti-K562 antibodies and complement, myeloid or erythroid colony formation was inhibited. Some of the inhibitory antibodies showed little binding to mature blood cells by radioimmunoassay, immunofluorescence, and complement cytotoxicity, suggesting that they recognize antigens specific to undifferentiated cells. With the fluorescence-activated cell sorter, one inhibitory antibody was shown to stain only 3% of bone marrow cells. Inhibitory anti-K562 antibodies also bind to myelogenous leukemia cells and virus-transformed lymphocytes. Thus, these antibodies appear to recognize antigens shared by normal hematopoietic progenitors, leukemic cells, and transformed lymphocytes.