PHOSPHOTYROSINE-CONTAINING PROTEINS ARE CONCENTRATED IN FOCAL ADHESIONS AND INTERCELLULAR-JUNCTIONS IN NORMAL-CELLS
PHOSPHOTYROSINE-CONTAINING PROTEINS ARE CONCENTRATED IN FOCAL ADHESIONS AND INTERCELLULAR-JUNCTIONS IN NORMAL-CELLS
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DOI：
10.1073/pnas.82.19.6576
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
SINGER, SJ
中科院分区：
文献类型：
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作者：
MAHER, PA;PASQUALE, EB;SINGER, SJ
我们使用针对磷酸酪氨酸 (P-Tyr) 的高亲和力多克隆抗体，通过免疫荧光显微镜实验定位正常和转化细胞中含有 P-Tyr 的蛋白质。将这些细胞中酪氨酸修饰蛋白的分布与 F-肌动蛋白的分布进行比较。正如预期的那样，发现感染阿贝尔森鼠白血病病毒的细胞中 P-Tyr 水平升高。成纤维细胞和上皮细胞的各种永久系表现出较低但易于检测的 P-Tyr 水平。成纤维细胞中的 P-Tyr 集中在含肌动蛋白的微丝束末端的焦点接触处，而在所检查的上皮细胞中，则集中在细胞间连接处。鸡胚成纤维细胞和鸡胚心脏细胞原代培养物的早期传代也显示出在焦点接触和细胞-细胞连接处可检测到的 P-Tyr 水平。然而，在鸡胚成纤维细胞的后续传代中未检测到 P-Tyr。正常细胞细胞间连接处含有 P-Tyr 的蛋白质浓度表明，这些是具有重要生化调节活性的位点，可能对控制正常细胞粘附性、运动性和形状很重要。
We have used a high-affinity polyclonal antibody directed against phosphotyrosine (P-Tyr) to localize P-Tyr-containing proteins in normal and transformed cells in culture by immunofluorescence microscopy experiments. The distribution of the proteins with modified tyrosine was compared with that of F-actin in these cells. Cells infected with Abelson murine leukemia virus were found to contain elevated levels of P-Tyr, as expected. Various permanent lines of fibroblastic and epithelial cells exhibited lower, but easily detectable, levels of P-Tyr. The P-Tyr in fibroblasts was concentrated at the focal contacts at the termini of actincontaining microfilament bundles and, in the epithelial cells examined, at the intercellular junctions. Early passages of primary cultures of chicken embryo fibroblasts and chicken embryo heart cells also showed detectable levels of P-Tyr in focal contacts and cell-cell junctions. However, P-Tyr was not detectable in later passages of chicken embryo fibroblasts. The concentration of P-Tyr-containing proteins in intercellular junctions in normal cells suggests that these are sites of significant biochemical regulatory activities which may be important in the control of normal cell adhesivity, motility, and shape.