Osteopontin expression is associated with hepatopathologic changes in Schistosoma japonicum infected mice
Osteopontin expression is associated with hepatopathologic changes in Schistosoma japonicum infected mice
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DOI：
10.3748/wjg.v17.i46.5075
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发表时间：
2011-12-14
影响因子：
4.3
通讯作者：
Li, Jia
中科院分区：
文献类型：
--
作者：
Chen, Bo-Lin;Zhang, Gui-Ying;Li, Jia
目的：探讨日本血吸虫感染BALB/C小鼠骨桥蛋白的表达及其与肝脏病理改变的关系。方法：采用腹腔感染血吸虫尾蚴法建立小鼠血吸虫肝病理模型。分别于感染后6、8、10、14和18周处死小鼠，取肝脏标本。苏木精-伊红、马松三色染色观察肝脏组织病理变化。采用免疫组织化学、逆转录聚合酶链反应和Western blotting检测骨桥蛋白的表达。免疫组化法检测小鼠α -平滑肌肌动蛋白（α - sma）和转化生长因子- β 1 （tgf - β 1）表达。分析骨桥蛋白表达与其他变量（α - sma、tgf - β 1、肝病理特征包括肉芽肿形成和肝纤维化程度）的相关性。结果：小鼠出现典型的血吸虫肝病理改变。第6周观察骨桥蛋白表达的动态变化。表达量升高，第10周达到高峰（P < 0.01），随后逐渐降低。骨桥蛋白表达与α - sma （r = 0.720, P < 0.01）、tgf - β 1 （r = 0.905, P < 0.01）、肉芽肿形成（r = 0.875, P < 0.01）、肝纤维化程度（r = 0.858, P < 0.01）呈正相关。结论：骨桥蛋白可能在血吸虫肝病理中起重要作用，并可能促进肉芽肿形成和肝纤维化，其机制尚不清楚。(C) 2011年白石登。版权所有。
AIM: To investigate osteopontin expression and its association with hepatopathologic changes in BALB/C mice infected with Schistosoma japonicum.METHODS: The schistosomal hepatopathologic mouse model was established by abdominal infection with schistosomal cercaria. Liver samples were obtained from mice sacrificed at 6, 8, 10, 14, and 18 wk after infection. Liver histopathological changes were observed with hematoxylin-eosin and Masson trichrome staining. The expression of osteopontin was determined with immunohistochemistry, reverse transcription-polymerase chain reaction, and Western blotting. The expression of alpha-smooth muscle actin (alpha-SMA) and transforming growth factor-beta 1 (TGF-beta 1) were determined by immunohistochemistry. Correlations of osteopontin expression with other variables (alpha-SMA, TGF-beta 1, hepatopathologic features including granuloma formation and degree of liver fibrosis) were analyzed.RESULTS: Typical schistosomal hepatopathologic changes were induced in the animals. Dynamic changes in the expression of osteopontin were observed at week 6. The expression increased, peaked at week 10 (P < 0.01), and then gradually decreased. Positive correlations between osteopontin expression and alpha-SMA (r = 0.720, P < 0.01), TGF-beta 1 (r = 0.905, P < 0.01), granuloma formation (r = 0.875, P < 0.01), and degree of liver fibrosis (r = 0.858, P < 0.01) were also observed.CONCLUSION: Osteopontin may play an important role in schistosomal hepatopathology and may promote granuloma formation and liver fibrosis through an unexplored mechanism. (C) 2011 Baishideng. All rights reserved.