Identification and discrimination of Burkholderia pseudomallei, B-mallei, and B-thailandensis by real-time PCR targeting type III secretion system genes
Identification and discrimination of Burkholderia pseudomallei, B-mallei, and B-thailandensis by real-time PCR targeting type III secretion system genes
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DOI：
10.1128/jcm.42.12.5871-5874.2004
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发表时间：
2004-12-01
影响因子：
9.4
通讯作者：
Vidal, DR
中科院分区：
文献类型：
--
作者：
Thibault, FM;Valade, E;Vidal, DR
鼻疽伯克霍尔德氏菌和鼻疽伯克霍尔德氏菌是两种高致病性细菌，分别导致类鼻疽和鼻疽。两者密切相关，也可能被误认为是非致病性物种 B. thailandensis。为了提高它们的差异鉴定，我们描述了一种基于水解探针的实时 PCR 方法，利用这三个物种中 III 型分泌系统基因的不均匀分布。
Burkholderia pseudomallei and B. mallei are two highly pathogenic bacteria, responsible for melioidosis and glanders, respectively. The two are closely related and can also be mistaken for B. thailandensis, a nonpathogenic species. To improve their differential identification, we describe a hydrolysis probe-based real-time PCR method using the uneven distribution of type III secretion system genes among these three species.