Utilization of iron-catecholamine complexes involving ferric reductase activity in Listeria monocytogenes
Utilization of iron-catecholamine complexes involving ferric reductase activity in Listeria monocytogenes
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DOI：
10.1128/iai.65.7.2778-2785.1997
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发表时间：
1997-07-01
影响因子：
3.1
通讯作者：
Vidon, DJM
中科院分区：
文献类型：
--
作者：
Coulanges, V;Andre, P;Vidon, DJM
单核细胞增多性李斯特菌是一种普遍存在的潜在致病微生物，生长和毒力需要铁，虽然II不产生铁载体，但单核细胞增多性李斯特菌能够利用各种微生物产生的外源铁载体或环境中广泛存在的天然邻苯二酚化合物获得铁，在铁螯合剂托洛酮的存在下，单核细胞增多性李斯特菌的生长被完全抑制。加入不同异构体的多巴胺或去甲肾上腺素可缓解其生长抑制作用，而儿茶酚胺类化合物4-羟基-3-甲氧基苯甘醇和去甲肾上腺素不能解除托波龙的抑制作用。用氯丙嗪和育亨宾预先孵育单核细胞增多性乳杆菌，不能拮抗儿茶酚胺在铁复合培养基中的促生长作用。此外，去甲肾上腺素对人转铁蛋白在铁限制条件下的促生长作用有明显的刺激作用。此外，多巴胺和去甲肾上腺素允许铁-55摄取存在于缺铁的细菌细胞中。ICON的摄取是能量依赖的，如在0℃抑制Fe-55摄取以及用KCN预培养细菌所表明的那样。在铂(II)存在的情况下，单核细胞增多性李斯特菌对Fe-55的摄取也受到抑制。此外，当用全细胞铁还原酶检测时，单核细胞增多性李斯特氏菌的还原酶活性被铂(II)抑制。这些数据表明，多巴胺和去甲肾上腺素可以作为铁载体类化合物在单核细胞增生性乳杆菌中发挥作用，这是因为它们具有邻二酚功能，而且儿茶酚胺介导的铁获取不涉及特定的儿茶酚胺受体，而是通过细胞结合的铁还原酶活性发挥作用。
Listeria monocytogenes is a ubiquitous potentially pathogenic organism requiring iron for growth and virulence, Although ii does not produce siderophores, L. monocytogenes is able to obtain iron by using either exogenous siderophores produced by various microorganisms or natural catechol compounds widespread in the environment, In the presence of tropolone, an iron-chelating agent, growth of L. monocytogenes is completely inhibited. However, the growth inhibition fan be relieved by the addition of dopamine or norepinephrine under their different isomeric forms, while the catecholamine derivatives 4-hydroxy-3-methoxyphenylglycol and normetanephrine did not relieve the inhibitory effect of tropolone. Preincubation of L. monocytogenes with chlorpromazine and yohimbine did not antagonize the growth-promoting effect of catecholamines in iron-complexed medium. In addition, norepinephrine stimulated the growth-promoting effect induced by human transferrin in iron-limited medium. Furthermore, dopamine and norepinephrine allowed Fe-55 uptake lay iron-deprived bacterial cells. The uptake Of icon was energy dependent, as indicated by inhibition of Fe-55 uptake at 0 degrees C as well as by preincubating the bacteria with KCN. Inhibition of Fe-55 uptake by L. monocytogenes was also observed in the presence of Pt(II). Moreover, when assessed by a whole-cell ferric reductase assay, reductase activity of L. monocytogenes was inhibited by Pt(II). These data demonstrate that dopamine and norepinephrine can function as siderophore-like compounds in L. monocytogenes owing to their ortho-diphenol function and that catecholamine-mediated iron acquisition does not involve specific catecholamine receptors but acts through a cell-bound ferrireductase activity.