One step carboxyl group isotopic labeling for quantitative analysis of intact N-glycopeptides by mass spectrometry.
One step carboxyl group isotopic labeling for quantitative analysis of intact N-glycopeptides by mass spectrometry.
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DOI：
10.1039/d1cc00197c
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发表时间：
2021-04
影响因子：
4.9
通讯作者：
Zhenyu Sun;G. Ji;Guoli Wang;Lei Wei;Ying Zhang;Haojie Lu
中科院分区：
文献类型：
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作者：
Zhenyu Sun;G. Ji;Guoli Wang;Lei Wei;Ying Zhang;Haojie Lu
在这里，我们已经开发了一种称为甲胺稳定同位素标记（MeSIL）的方法，通过一步标记相对定量N-糖肽。这是首次将该方法应用于测量寨卡病毒感染后huh 7细胞中N-糖肽的变化，并且已经揭示了差异表达的N-糖肽在糖基化位点特异性水平上在病毒感染中起重要作用。
Here, we have developed an approach termed methylamine stable isotope labeling (MeSIL) to relatively quantify N-glycopeptides through one step labeling. It is the first time that this approach is applied to measure N-glycopeptide changes in huh7 cells after Zika virus infection and it has been revealed that differentially expressed N-glycopeptides played important roles in virus infection at the glycosylation site-specific level.