Preliminary crystallographic study of protocatechuate 3,4-dioxygenase from Brevibacterium fuscum.
Preliminary crystallographic study of protocatechuate 3,4-dioxygenase from Brevibacterium fuscum.
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褐色短杆菌原儿茶酸 3,4-双加氧酶的初步晶体学研究。
DOI：
10.1006/jmbi.1994.1143
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发表时间：
1994
影响因子：
5.6
通讯作者：
Ohlendorf,DH
中科院分区：
文献类型：
--
作者：
Earhart,CA;Radhakrishnan,R;Orville,AM;Lipscomb,JD;Ohlendorf,DH
来自革兰氏阳性菌的原儿茶酸3，4-双加氧酶属于三斜晶系空间群P1，晶胞尺寸为a=96？1？，b=97？2？，c=118？1？，α=113.9°，β=90.7°，γ=117.8°。棒状晶体的衍射率达到2.4？分辨率。旋转函数分析表明，在不对称单元中存在6个具有局部32对称性的原型，而不是先前提出的五聚体复合体。
The enzyme protocatechuate 3,4-dioxygenase from the Gram positive organismBrevibacterium fuscumcrystallizes in the triclinic space groupP1 with unit cell dimensionsa= 96·1 Å,b= 97·2 Å,c= 118·1 Å and α = 113·9°, β = 90·7°, γ = 117·8°. The rod-like crystals diffract to 2·4 Å resolution. Rotation function analysis suggests that there are six protomers arranged with local 32 symmetry in the asymmetric unit rather than the previously proposed pentameric complex.